Publication detail

Particle-based immunochemical separation of methicillin resistant: Staphylococcus aureus with indirect electrochemical detection of labeling oligonucleotides

ČÍHALOVÁ, K. HEGEROVÁ, D. DOSTÁLOVÁ, S. JELÍNKOVÁ, P. KREJČOVÁ, L. MILOSAVLJEVIĆ, V. KŘÍŽKOVÁ, S. KOPEL, P. ADAM, V.

Original Title

Particle-based immunochemical separation of methicillin resistant: Staphylococcus aureus with indirect electrochemical detection of labeling oligonucleotides

Type

journal article in Web of Science

Language

English

Original Abstract

Early detection of antibiotic-resistant bacteria causing inflammation in patients is a key for an appropriate and timely treatment. Classical microbiological cultivation methods are time-consuming and therefore it is necessary to discover a quick and effective method. The method used in this study is based on a sandwich immunoreaction of bacteria with IgG immobilized on streptavidin-coated polystyrene superparamagnetic particles and MRSA-specific antibody immobilized on gold non-magnetic nanoparticles labeled with oligonucleotides, which were detected by square wave voltammetry in combination with the adsorptive transfer technique. The assay is suitable for selective detection of methicillin-resistant Staphylococcus aureus in samples even at a concentration of 2  104 CFU mL1. The method can be flexibly modified for any other bacteria, depending on the antibodies used.

Keywords

Staphylococcus aureus, electrochemical detection, MRSA, antibody

Authors

ČÍHALOVÁ, K.; HEGEROVÁ, D.; DOSTÁLOVÁ, S.; JELÍNKOVÁ, P.; KREJČOVÁ, L.; MILOSAVLJEVIĆ, V.; KŘÍŽKOVÁ, S.; KOPEL, P.; ADAM, V.

Released

7. 6. 2016

ISBN

1759-9679

Periodical

Analytical Methods

Year of study

8

Number

25

State

United Kingdom of Great Britain and Northern Ireland

Pages from

5123

Pages to

5128

Pages count

6

BibTex

@article{BUT126820,
  author="Kristýna {Číhalová} and Dagmar {Hegerová} and Simona {Dostálová} and Pavlína {Jelínková} and Ludmila {Krejčová} and Vedran {Milosavljević} and Soňa {Křížková} and Pavel {Kopel} and Vojtěch {Adam}",
  title="Particle-based immunochemical separation of methicillin resistant: Staphylococcus aureus with indirect electrochemical detection of labeling oligonucleotides",
  journal="Analytical Methods",
  year="2016",
  volume="8",
  number="25",
  pages="5123--5128",
  doi="10.1039/c6ay01296e",
  issn="1759-9679"
}