Publication detail

Nanoparticles Suitable for BCAA Isolation Can Serve for Use in Magnetic Lipoplex-Based Delivery System for L, I, V, or R-rich Antimicrobial Peptides

VESELÝ, R. JELÍNKOVÁ, P. HEGEROVÁ, D. CERNEI, N. KOPEL, P. MOULICK, A. RICHTERA, L. HEGER, Z. ADAM, V. ZÍTKA, O.

Original Title

Nanoparticles Suitable for BCAA Isolation Can Serve for Use in Magnetic Lipoplex-Based Delivery System for L, I, V, or R-rich Antimicrobial Peptides

Type

journal article in Web of Science

Language

English

Original Abstract

This paper investigates the synthesis of paramagnetic nanoparticles, which are able to bind branched chain amino acids (BCAAs)—leucine, valine, and isoleucine and, thus, serve as a tool for their isolation. Further, by this, we present an approach for encapsulation of nanoparticles into a liposome cavity resulting in a delivery system. Analyses of valine and leucine in entire complex show that 31.3% and 32.6% recoveries are reached for those amino acids. Evaluation of results shows that the success rate of delivery in Escherichia coli (E. coli) is higher in the case of BCAAs on nanoparticles entrapped in liposomes (28.7% and 34.7% for valine and leucine, respectively) when compared to nanoparticles with no liposomal envelope (18.3% and 13.7% for valine and leucine, respectively). The nanoparticles with no liposomal envelope exhibit the negative zeta potential ( 9.1 0.3 mV); however, their encapsulation results in a shift into positive values (range of 28.9 0.4 to 33.1 0.5 mV). Thus, electrostatic interactions with negatively-charged cell membranes (approx. 50 mV in the case of E. coli) leads to a better uptake of cargo. Our delivery system was finally tested with the leucine-rich antimicrobial peptide (FALALKALKKALKKLKKALKKAL) and it is shown that hemocompatibility (7.5%) and antimicrobial activity of the entire complex against E. coli, Staphylococcus aureus (S. aureus), and methicilin-resistant S. aureus (MRSA) is comparable or better than conventional penicillin antibiotics.

Keywords

branched chain amino acids; encapsulation; Escherichia coli; nanomedicine; Staphylococcus aureus

Authors

VESELÝ, R.; JELÍNKOVÁ, P.; HEGEROVÁ, D.; CERNEI, N.; KOPEL, P.; MOULICK, A.; RICHTERA, L.; HEGER, Z.; ADAM, V.; ZÍTKA, O.

Released

1. 4. 2016

Publisher

MDPI

ISBN

1996-1944

Periodical

Materials

Year of study

9

Number

4

State

Swiss Confederation

Pages from

260-1

Pages to

260-15

Pages count

15

URL

http://dx.doi.org/10.3390/ma9040260

Full text in the Digital Library

http://hdl.handle.net/11012/63815