Publication detail

Specific Magnetic Isolation of E6 HPV16 Modified Magnetizable Particles Coupled with PCR and Electrochemical Detection

JIMENEZ JIMENEZ, A. RUTTKAY-NEDECKÝ, B. DOSTÁLOVÁ, S. KREJČOVÁ, L. MICHÁLEK, P. RICHTERA, L. ADAM, V.

Original Title

Specific Magnetic Isolation of E6 HPV16 Modified Magnetizable Particles Coupled with PCR and Electrochemical Detection

Type

journal article in Web of Science

Language

English

Original Abstract

The majority of carcinomas that were developed due to the infection with human papillomavirus (HPV) are caused by high-risk HPV types, HPV16 and HPV18. These HPV types contain the E6 and E7 oncogenes, so the fast detection of these oncogenes is an important point to avoid the development of cancer. Many different HPV tests are available to detect the presence of HPV in biological samples. The aim of this study was to design a fast and low cost method for HPV identification employing magnetic isolation, polymerase chain reaction (PCR) and electrochemical detection. These assays were developed to detect the interactions between E6-HPV16 oncogene and magnetizable particles (MPs) using commercial Dynabeads M-280 Streptavidin particles and laboratory-synthesized “homemade” particles called MANs (MAN-37, MAN-127 and MAN-164). The yields of PCR amplification of E6-HPV16 oncogene bound on the particles and after the elution from the particles were compared. A highest yield of E6-HPV16 DNA isolation was obtained with both MPs particles commercial M-280 Streptavidin and MAN-37 due to reducing of the interferents compared with the standard PCR method. A biosensor employing the isolation of E6-HPV16 oncogene with MPs particles followed by its electrochemical detection can be a very effective technique for HPV identification, providing simple, sensitive and cost-effective analysis.

Keywords

electrochemistry; human papillomavirus; magnetic isolation; nucleic acid detection; magnetizable particles; PCR

Authors

JIMENEZ JIMENEZ, A.; RUTTKAY-NEDECKÝ, B.; DOSTÁLOVÁ, S.; KREJČOVÁ, L.; MICHÁLEK, P.; RICHTERA, L.; ADAM, V.

Released

1. 5. 2016

Publisher

MDPI

ISBN

1422-0067

Periodical

International Journal of Molecular Sciences

Year of study

17

Number

5

State

Swiss Confederation

Pages from

1

Pages to

15

Pages count

15

URL

http://www.mdpi.com/1422-0067/17/5/585

Full text in the Digital Library

http://hdl.handle.net/11012/63332

BibTex

@article{BUT127856,
  author="Ana Maria {Jimenez Jimenez} and Branislav {Ruttkay-Nedecký} and Simona {Dostálová} and Ludmila {Krejčová} and Lukáš {Richtera} and Vojtěch {Adam} and Petr {Michálek}",
  title="Specific Magnetic Isolation of E6 HPV16 Modified Magnetizable Particles Coupled with PCR and Electrochemical Detection",
  journal="International Journal of Molecular Sciences",
  year="2016",
  volume="17",
  number="5",
  pages="1--15",
  doi="10.3390/ijms17050585",
  issn="1422-0067",
  url="http://www.mdpi.com/1422-0067/17/5/585"
}