Publication detail

MATRA-A reagent/polyethyleneimine complex transfection for low toxic DNA plasmid delivery

SVOBODA, O. FOHLEROVÁ, Z. BAIAZITOVA, L. ČMIEL, V. HUBÁLEK, J. PROVAZNÍK, I. PEKAŘÍK, V.

Original Title

MATRA-A reagent/polyethyleneimine complex transfection for low toxic DNA plasmid delivery

Type

article in a collection out of WoS and Scopus

Language

English

Original Abstract

The non-toxic intracellular delivery of biopolymers is very hot topic in current biology. In this work, two different MATRA-A reagent/polyethyleneimine transfection protocols were used for intracellular delivery of ChR2 DNA vector. Cell morphology changes were studied 48 hours after transfection. Our transfection efficiency is lower than normally achieved, but the rate of cells with physiological properties remains is very high: 85.34%. Therefore, these transfection protocols are very applicable for electrophysiological experiments.

Keywords

transfection, MATRA-A reagent, plasmid delivery

Authors

SVOBODA, O.; FOHLEROVÁ, Z.; BAIAZITOVA, L.; ČMIEL, V.; HUBÁLEK, J.; PROVAZNÍK, I.; PEKAŘÍK, V.

Released

6. 5. 2016

Publisher

Masarykova Univerzita - MUNI PRESS

Location

Brno, Czech Republic

ISBN

978-80-210-8267-0

Book

XVI. Workshop of Physical Chemists and Electrochemists

Edition

1

Pages from

1

Pages to

3

Pages count

3

BibTex

@inproceedings{BUT128268,
  author="Ondřej {Svoboda} and Zdenka {Fohlerová} and Larisa {Chmelíková} and Vratislav {Čmiel} and Jaromír {Hubálek} and Valentine {Provazník} and Vladimír {Pekařík}",
  title="MATRA-A reagent/polyethyleneimine complex transfection for low toxic DNA plasmid delivery",
  booktitle="XVI. Workshop of Physical Chemists and Electrochemists",
  year="2016",
  series="1",
  pages="1--3",
  publisher="Masarykova Univerzita - MUNI PRESS",
  address="Brno, Czech Republic",
  isbn="978-80-210-8267-0"
}