Publication detail

Fluorescence Characterization of Gold Modified Liposomes with Antisense N-myc DNA Bound to the Magnetisable Particles with Encapsulated Anticancer Drugs (Doxorubicin, Ellipticine and Etoposide)

SKALIČKOVÁ, S. NEJDL, L. KUDR, J. RUTTKAY-NEDECKÝ, B. JIMENEZ JIMENEZ, A. KOPEL, P. KREMPLOVÁ, M. MASAŘÍK, M. STIBOROVÁ, M. ECKSCHLAGER, T. ADAM, V. KIZEK, R.

Original Title

Fluorescence Characterization of Gold Modified Liposomes with Antisense N-myc DNA Bound to the Magnetisable Particles with Encapsulated Anticancer Drugs (Doxorubicin, Ellipticine and Etoposide)

Type

journal article in Web of Science

Language

English

Original Abstract

Liposome-based drug delivery systems hold great potential for cancer therapy. The aim of this study was to design a nanodevice for targeted anchoring of liposomes (with and without cholesterol) with encapsulated anticancer drugs and antisense N-myc gene oligonucleotide attached to its surface. To meet this main aim, liposomes with encapsulated doxorubicin, ellipticine and etoposide were prepared. They were further characterized by measuring their fluorescence intensity, whereas the encapsulation efficiency was estimated to be 16%. The hybridization process of individual oligonucleotides forming the nanoconstruct was investigated spectrophotometrically and electrochemically. The concentrations of ellipticine, doxorubicin and etoposide attached to the nanoconstruct in gold nanoparticle-modified liposomes were found to be 14, 5 and 2 µg•mL(-1), respectively. The study succeeded in demonstrating that liposomes are suitable for the transport of anticancer drugs and the antisense oligonucleotide, which can block the expression of the N-myc gene.

Keywords

liposome; gold nanoparticles; N-myc; doxorubicin; ellipticine; etoposide

Authors

SKALIČKOVÁ, S.; NEJDL, L.; KUDR, J.; RUTTKAY-NEDECKÝ, B.; JIMENEZ JIMENEZ, A.; KOPEL, P.; KREMPLOVÁ, M.; MASAŘÍK, M.; STIBOROVÁ, M.; ECKSCHLAGER, T.; ADAM, V.; KIZEK, R.

Released

1. 3. 2016

Publisher

MDPI

ISBN

1424-8220

Periodical

SENSORS

Year of study

16

Number

3

State

Swiss Confederation

Pages from

1

Pages to

11

Pages count

11

URL

http://www.mdpi.com/1424-8220/16/3/290

Full text in the Digital Library

http://hdl.handle.net/11012/63728

BibTex

@article{BUT128498,
  author="Sylvie {Skaličková} and Lukáš {Nejdl} and Jiří {Kudr} and Branislav {Ruttkay-Nedecký} and Ana Maria {Jimenez Jimenez} and Pavel {Kopel} and Monika {Kremplová} and Michal {Masařík} and Marie {Stiborová} and Tomáš {Eckschlager} and Vojtěch {Adam} and René {Kizek}",
  title="Fluorescence Characterization of Gold Modified Liposomes with Antisense N-myc DNA Bound to the Magnetisable Particles with Encapsulated Anticancer Drugs (Doxorubicin, Ellipticine and Etoposide)",
  journal="SENSORS",
  year="2016",
  volume="16",
  number="3",
  pages="1--11",
  doi="10.3390/s16030290",
  issn="1424-8220",
  url="http://www.mdpi.com/1424-8220/16/3/290"
}