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PEJOVIC SIMEUNOVIC, J. PEKÁRKOVÁ, J. ŽAK, J. HUBÁLEK, J.
Original Title
ON-A-CHIP ELECTROPHORESIS FOR THE CHARACTERIZATION OF QUANTUM DOTS BIOCONJUGATES
Type
conference paper
Language
English
Original Abstract
On a chip electrophoresis was used to characterize quantum dots (QDs) and their conjugates with biological molecules. Due to very diverse and numerous applications of QDs, it is really important to make a tool for precise and controlled detection of QDs. In this study, we investigate on a chip detection and separation of QDs bioconjugated with BSA (bovine serum albumin) using homemade equipment based on principle of capillary electrophoresis (CE) and optical detection. Satisfactory separation of complex from free QDs was achieved with 10mM Borate Buffered Saline (BBS) aqueous solution (pH 9.14), as a separation buffer, within 10min.
Keywords
quantum dots, separation, protein, electrophoresis
Authors
PEJOVIC SIMEUNOVIC, J.; PEKÁRKOVÁ, J.; ŽAK, J.; HUBÁLEK, J.
Released
17. 10. 2016
Publisher
Institute of Analytical Chemistry of the CAS
Location
Brno
ISBN
978-80-904959-4-4
Book
CECE 2016, the 13 th International Interdisciplinary Meeting on Bioanalysis
Pages from
211
Pages to
214
Pages count
4
BibTex
@inproceedings{BUT130681, author="Jelena {Pejović Simeunović} and Jana {Pekárková} and Jaromír {Žák} and Jaromír {Hubálek}", title="ON-A-CHIP ELECTROPHORESIS FOR THE CHARACTERIZATION OF QUANTUM DOTS BIOCONJUGATES", booktitle="CECE 2016, the 13 th International Interdisciplinary Meeting on Bioanalysis", year="2016", pages="211--214", publisher="Institute of Analytical Chemistry of the CAS", address="Brno", isbn="978-80-904959-4-4" }