Publication detail

Induction of Laccase, Lignin Peroxidase and Manganese Peroxidase Activities in White-Rot Fungi Using Copper Complexes

VRŠANSKÁ, M. VOBĚRKOVÁ, S. LANGER, V. PALOVČÍKOVÁ, D. MOULICK, A. ADAM, V. KOPEL, P.

Original Title

Induction of Laccase, Lignin Peroxidase and Manganese Peroxidase Activities in White-Rot Fungi Using Copper Complexes

Type

journal article in Web of Science

Language

English

Original Abstract

Ligninolytic enzymes, such as laccase, lignin peroxidase and manganese peroxidase, are biotechnologically-important enzymes. The ability of five white-rot fungal strains Daedaleopsis confragosa, Fomes fomentarius, Trametes gibbosa, Trametes suaveolens and Trametes versicolor to produce these enzymes has been studied. Three different copper(II) complexes have been prepared ((Him)[Cu(im)4(H2O)2](btc) 3H2O, where im = imidazole, H3btc = 1,3,5-benzenetricarboxylic acid, [Cu3(pmdien)3(btc)](ClO4)3 6H2O) and [Cu3(mdpta)3(btc)](ClO4)3 4H2O, where pmdien = N,N,N0,N0 0, N0 0-pentamethyl-diethylenetriamine and mdpta = N,N-bis-(3-aminopropyl)methyl-amine), and their potential application for laccase and peroxidases induction have been tested. The enzyme-inducing activities of the complexes were compared with that of copper sulfate, and it has been found that all of the complexes are suitable for the induction of laccase and peroxidase activities in white-rot fungi; however, the newly-synthesized complex M1 showed the greatest potential for the induction. With respect to the different copper inducers, this parameter seems to be important for enzyme activity, which depends also on the fungal strains.

Keywords

laccase; peroxidase; copper complex; trimesic acid; enzymatic induction

Authors

VRŠANSKÁ, M.; VOBĚRKOVÁ, S.; LANGER, V.; PALOVČÍKOVÁ, D.; MOULICK, A.; ADAM, V.; KOPEL, P.

Released

1. 11. 2016

Publisher

MDPI

ISBN

1420-3049

Periodical

MOLECULES

Year of study

21

Number

11

State

Swiss Confederation

Pages from

1

Pages to

15

Pages count

15

URL

http://www.mdpi.com/1420-3049/21/11/1553

Full text in the Digital Library

http://hdl.handle.net/11012/69925

BibTex

@article{BUT131468,
  author="Martina {Vršanská} and Stanislava {Voběrková} and Vratislav {Langer} and Dagmar {Palovčíková} and Amitava {Moulick} and Vojtěch {Adam} and Pavel {Kopel}",
  title="Induction of Laccase, Lignin Peroxidase and Manganese Peroxidase Activities in White-Rot Fungi Using Copper Complexes",
  journal="MOLECULES",
  year="2016",
  volume="21",
  number="11",
  pages="1--15",
  doi="10.3390/molecules21111553",
  issn="1420-3049",
  url="http://www.mdpi.com/1420-3049/21/11/1553"
}