Publication detail

Sarcosine up-regulates expression of genes involved in cell cycle progression of metastatic models of prostate cancer

HEGER, Z. MERLOS RODRIGO, M. MICHÁLEK, P. POLANSKÁ, H. MASAŘÍK, M. VÍT, V. PLEVOVÁ, M. PACÍK, D. ECKSCHLAGER, T. STIBOROVÁ, M. ADAM, V.

Original Title

Sarcosine up-regulates expression of genes involved in cell cycle progression of metastatic models of prostate cancer

Type

journal article in Web of Science

Language

English

Original Abstract

The effects of sarcosine on the processes driving prostate cancer (PCa) development remain still unclear. Herein, we show that a supplementation of metastatic PCa cells (androgen independent PC-3 and androgen dependent LNCaP) with sarcosine stimulates cells proliferation in vitro. Similar stimulatory effects were observed also in PCa murine xenografts, in which sarcosine treatment induced a tumor growth and significantly reduced weight of treated mice (p < 0.05). Determination of sarcosine metabolism-related amino acids and enzymes within tumor mass revealed significantly increased glycine, serine and sarcosine concentrations after treatment accompanied with the increased amount of sarcosine dehydrogenase. In both tumor types, dimethylglycine and glycine-N-methyltransferase were affected slightly, only. To identify the effects of sarcosine treatment on the expression of genes involved in any aspect of cancer development, we further investigated expression profiles of excised tumors using cDNA electrochemical microarray followed by validation using the semi-quantitative PCR. We found 25 differentially expressed genes in PC-3, 32 in LNCaP tumors and 18 overlapping genes. Bioinformatical processing revealed strong sarcosine-related induction of genes involved particularly in a cell cycle progression. Our exploratory study demonstrates that sarcosine stimulates PCa metastatic cells irrespectively of androgen dependence. Overall, the obtained data provides valuable information towards understanding the role of sarcosine in PCa progression and adds another piece of puzzle into a picture of sarcosine oncometabolic potential.

Keywords

metabolism-related proteins; one-carbon metabolism; thymidylate synthase; breast-cancer; phosphorylation; proliferation; receptors; therapy; glycine; serine

Authors

HEGER, Z.; MERLOS RODRIGO, M.; MICHÁLEK, P.; POLANSKÁ, H.; MASAŘÍK, M.; VÍT, V.; PLEVOVÁ, M.; PACÍK, D.; ECKSCHLAGER, T.; STIBOROVÁ, M.; ADAM, V.

Released

8. 11. 2016

Publisher

PLOS

ISBN

1932-6203

Periodical

PLOS ONE

Year of study

11

Number

11

State

United States of America

Pages from

1

Pages to

20

Pages count

20

URL

http://europepmc.org/articles/PMC5100880?pdf=render

Full text in the Digital Library

http://hdl.handle.net/11012/69380

BibTex

@article{BUT131991,
  author="Zbyněk {Heger} and Miguel Ángel {Merlos Rodrigo} and Petr {Michálek} and Hana {Polanská} and Michal {Masařík} and Vítězslav {Vít} and Mariana {Plevová} and Dalibor {Pacík} and Tomáš {Eckschlager} and Marie {Stiborová} and Vojtěch {Adam}",
  title="Sarcosine up-regulates expression of genes involved in cell cycle progression of metastatic models of prostate cancer",
  journal="PLOS ONE",
  year="2016",
  volume="11",
  number="11",
  pages="1--20",
  doi="10.1371/journal.pone.0165830",
  issn="1932-6203",
  url="http://europepmc.org/articles/PMC5100880?pdf=render"
}