Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration

journal article in Web of Science

English

We describe a new procedure for the parallel mapping of selected metals in histologically characterized tissue samples. Mapping is achieved via image registration of digital data obtained from two neighbouring cryosections by scanning the first as a histological sample and subjecting the second to laser ablation inductively coupled plasma mass spectrometry. This computer supported procedure enables determination of the distribution and content of metals of interest directly in the chosen histological zones and represents a substantial improvement over the standard approach, which determines these values in tissue homogenates or whole tissue sections. The potential of the described procedure was demonstrated in a pilot study that analysed Zn and Cu levels in successive development stages of pig melanoma tissue using MeLiM (Melanoma-bearing-Libechov-Minipig) model. We anticipate that the procedure could be useful for a complex understanding of the role that the spatial distribution of metals plays within tissues affected by pathological states including cancer.

LA-ICP-MS; BEARING LIBECHOV MINIPIG; MIXED-EFFECTS MODELS; METALLOTHIONEIN-OVEREXPRESSION; MELANOMA PATIENTS; ZINC LEVELS; COPPER; MELANOBLASTOMA; ELEMENTS; STRESS

ANÝZ, J.; VYSLOUŽILOVÁ, L.; VACULOVIČ, T.; TVRDOŇOVÁ, M.; KANICKÝ, V.; HAASE, H.; HORÁK, V.; ŠTĚPÁNKOVÁ, O.; HEGER, Z.; ADAM, V.

10. 1. 2017

Springer Nature

2045-2322

Scientific Reports

7

40169

United Kingdom of Great Britain and Northern Ireland

40169-1

40169-13

13

https://www.nature.com/articles/srep40169

http://hdl.handle.net/11012/69381