Publication detail

Monitoring of quantum dot distribution in plants by in vivo fluorescence imaging

VANĚČKOVÁ, T. MILOSAVLJEVIĆ, V. KRYŠTOFOVÁ, O. ADAM, V. VACULOVIČOVÁ, M.

Original Title

Monitoring of quantum dot distribution in plants by in vivo fluorescence imaging

Type

abstract

Language

English

Original Abstract

Nanoparticle visualization has extensive potential for their utilization in targeted drug delivery tracking through wide range of biological environments. In this regard, quantum dot nanoparticles may represent a suitable option as they are characterized by very good fluorescent properties. This study is focused on application of quantum dot nanoparticles for fluorescence imaging of plants. A series of in vivo experiments was carried out on leaves of sunflower plant (Helianthus annuus). Leaves were immersed in water solution of CdTe and CdTe/ZnS quantum dots and were monitored (ʎem 535 nm and 600 nm respectively) for 8 hours at time intervals of 60 minutes using In Vivo Xtreme Imaging System (Bruker, Massachusets, USA). Captured images were further processed and the intensity of fluorescence analyzed by Bruker Molecular Imaging Software. Fluorescence imaging of plants encounters difficulties due to autofluorescence of biomolecules present in plants, including chlorophyll, carotene and xanthophyll. However, this can be suppressed by using adequate excitation and emission filters for fluorescence images acquisition. Moreover, visualization of nanoparticles in plants can be enhanced by multispectral imaging and spectral modeling for autofluorescence unmixing. In this study, evaluation regarding optimal characteristics and modifications of quantum dot nanoparticles for fluorescence imaging in plants is provided as well as recommendations on image acquisition setting and further image postprocessing.

Keywords

QDs; in VIVO; fluorescence; nanoparticles

Authors

VANĚČKOVÁ, T.; MILOSAVLJEVIĆ, V.; KRYŠTOFOVÁ, O.; ADAM, V.; VACULOVIČOVÁ, M.

Released

21. 10. 2016

Publisher

AMOS repro Ltd.

Location

Ostrava, Czech Republic

ISBN

978-80-87294-68-0

Book

NANOCON 2016

Pages from

112

Pages to

112

Pages count

1

BibTex

@misc{BUT133444,
  author="Tereza {Vaněčková} and Vedran {Milosavljević} and Olga {Kryštofová} and Vojtěch {Adam} and Markéta {Vaculovičová}",
  title="Monitoring of quantum dot distribution in plants by in vivo fluorescence imaging",
  booktitle="NANOCON 2016",
  year="2016",
  pages="112--112",
  publisher="AMOS repro Ltd.",
  address="Ostrava, Czech Republic",
  isbn="978-80-87294-68-0",
  note="abstract"
}