Publication detail

Computer-Assisted Engineering of Synthetic Pathway for Biodegradation of Toxic Persistent Pollutant

KURUMBANG, N. DVOŘÁK, P. BENDL, J. BREZOVSKÝ, J. PROKOP, Z. DAMBORSKÝ, J.

Original Title

Computer-Assisted Engineering of Synthetic Pathway for Biodegradation of Toxic Persistent Pollutant

Type

journal article in Web of Science

Language

English

Original Abstract

Anthropogenic halogenated compounds had been unknown to the nature until industrial revolution and microorganisms have not had sufficient time to evolve enzymes for their degradation. The lack of efficient enzymes and natural metabolic pathways can be addressed by combination of protein and metabolic engineering. We have assembled a synthetic pathway for conversion of highly toxic and recalcitrant 1,2,3-trichloropropane to glycerol in Escherichia coli and used it for systematic study of pathway bottlenecks. The optimal ratios of enzymes for maximal production of glycerol and minimal toxicity of metabolites were predicted using a mathematical model. The strains containing predicted ratios of enzymes were constructed and characterized for their viability and degradation efficiency. An excellent agreement between predicted and experimental data has been observed. The validated model has been used to quantitatively describe the kinetic limitations of currently available enzyme variants and predict the improvements required for further pathway optimization. Presented concept highlights the potential of rigorous rational engineering of microorganisms for degradation of toxic anthropogenic compounds.

Keywords

activity, enantioselectivity, kinetic modeling, protein and metabolic engineering, synthetic pathway, toxicity

Authors

KURUMBANG, N.; DVOŘÁK, P.; BENDL, J.; BREZOVSKÝ, J.; PROKOP, Z.; DAMBORSKÝ, J.

RIV year

2014

Released

21. 3. 2014

ISBN

2161-5063

Periodical

ACS Synthetic Biology

Year of study

3

Number

3

State

United States of America

Pages from

172

Pages to

181

Pages count

10

URL

http://pubs.acs.org/doi/pdf/10.1021/sb400147n

BibTex

@article{BUT133483,
  author="Nagendra {Kurumbang} and Pavel {Dvořák} and Jaroslav {Bendl} and Jan {Brezovský} and Zbyněk {Prokop} and Jiří {Damborský}",
  title="Computer-Assisted Engineering of Synthetic Pathway for Biodegradation of Toxic Persistent Pollutant",
  journal="ACS Synthetic Biology",
  year="2014",
  volume="3",
  number="3",
  pages="172--181",
  doi="10.1021/sb400147n",
  issn="2161-5063",
  url="http://pubs.acs.org/doi/pdf/10.1021/sb400147n"
}

Documents

acs_2013.pdf