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KOMÍNKOVÁ, M. MILOSAVLJEVIĆ, V. VÍTEK, P. POLANSKÁ, H. ČÍHALOVÁ, K. DOSTÁLOVÁ, S. HYNŠTOVÁ, V. GURÁŇ, R. KOPEL, P. RICHTERA, L. MASAŘÍK, M. BRTNICKÝ, M. KYNICKÝ, J. ZÍTKA, O. ADAM, V.
Original Title
Comparative study on toxicity of extracellularly biosynthesized and laboratory synthesized CdTe quantum dots
Type
journal article in Web of Science
Language
English
Original Abstract
Nanobiosynthesis belongs to the most recent methods for synthesis of nanoparticles. This type of syn-thesis provides many advantages including the uniformity in particle shape and size. The biosynthesishas also a significant advantage regarding chemical properties of the obtained particles. In this study, wecharacterized the basic properties and composition of quantum dots (QDs), obtained by the extracellularbiosynthesis by Escherichia coli. Furthermore, the toxicity of the biosynthesized QDs was compared toQDs prepared by microwave synthesis. The obtained results revealed the presence of cyan CdTe QDs afterremoval of substantial amounts of organic compounds, which stabilized the nanoparticle surface. QDstoxicity was evaluated using three cell lines Human Foreskin Fibroblast (HFF), Human Prostate Cancercells (PC-3) and Breast Cancer cells (MCF-7) and the MTT assay. The test revealed differences in the toxic-ity between variants of QDs, varying about 10% in the HFF and 30% in the MCF-7 cell lines. The toxicity ofthe biosynthesized QDs to the PC-3 cell lines was about 35% lower in comparison with the QDs preparedby microwave synthesis.
Keywords
Quantum dots; Biosynthesis; Escherichia coli (E. coli); CdTeToxicity
Authors
KOMÍNKOVÁ, M.; MILOSAVLJEVIĆ, V.; VÍTEK, P.; POLANSKÁ, H.; ČÍHALOVÁ, K.; DOSTÁLOVÁ, S.; HYNŠTOVÁ, V.; GURÁŇ, R.; KOPEL, P.; RICHTERA, L.; MASAŘÍK, M.; BRTNICKÝ, M.; KYNICKÝ, J.; ZÍTKA, O.; ADAM, V.
Released
10. 1. 2017
ISBN
0168-1656
Periodical
Journal of Biotechnology
Year of study
241
Number
1
State
Kingdom of the Netherlands
Pages from
193
Pages to
200
Pages count
8
BibTex
@article{BUT134072, author="KOMÍNKOVÁ, M. and MILOSAVLJEVIĆ, V. and VÍTEK, P. and POLANSKÁ, H. and ČÍHALOVÁ, K. and DOSTÁLOVÁ, S. and HYNŠTOVÁ, V. and GURÁŇ, R. and KOPEL, P. and RICHTERA, L. and MASAŘÍK, M. and BRTNICKÝ, M. and KYNICKÝ, J. and ZÍTKA, O. and ADAM, V.", title="Comparative study on toxicity of extracellularly biosynthesized and laboratory synthesized CdTe quantum dots", journal="Journal of Biotechnology", year="2017", volume="241", number="1", pages="193--200", doi="10.1016/j.jbiotec.2016.10.024", issn="0168-1656" }