Publication detail

Apoferritin as an ubiquitous nanocarrier with excellent shelf life

DOSTÁLOVÁ, S. VAŠÍČKOVÁ, K. HYNEK, D. KŘÍŽKOVÁ, S. RICHTERA, L. VACULOVIČOVÁ, M. ECKSCHLAGER, T. STIBOROVÁ, M. HEGER, Z. ADAM, V.

Original Title

Apoferritin as an ubiquitous nanocarrier with excellent shelf life

Type

journal article in Web of Science

Language

English

Original Abstract

Due to many adverse effects of conventional chemotherapy, novel methods of targeting drugs to cancer cells are being investigated. Nanosize carriers are a suitable platform for this specific delivery. Herein, we evaluated the long-term stability of the naturally found protein nanocarrier apoferritin (Apo) with encapsulated doxorubicin (Dox). The encapsulation was performed using Apo’s ability to disassemble reversibly into its subunits at low pH (2.7) and reassemble in neutral pH (7.2), physically entrapping drug molecules in its cavity (creating ApoDox). In this study, ApoDox was prepared in water and phosphate-buffered saline and stored for 12 weeks in various conditions (-20°C, 4°C, 20°C, and 37°C in dark, and 4°C and 20°C under ambient light). During storage, a very low amount of prematurely released drug molecules were detected (maximum of 7.5% for ApoDox prepared in PBS and 4.4% for ApoDox prepared in water). Fourier-transform infrared spectra revealed no significant differences in any of the samples after storage. Most of the ApoDox prepared in phosphate-buffered saline and ApoDox prepared in water and stored at -20°C formed very large aggregates (up to 487% of original size). Only ApoDox prepared in water and stored at 4°C showed no significant increase in size or shape. Although this storage caused slower internalization to LNCaP prostate cancer cells, ApoDox (2.5 μM of Dox) still retained its ability to inhibit completely the growth of 1.5×104 LNCaP cells after 72 hours. ApoDox stored at 20°C and 37°C in water was not able to deliver Dox inside the nucleus, and thus did not inhibit the growth of the LNCaP cells. Overall, our study demonstrates that ApoDox has very good stability over the course of 12 weeks when stored properly (at 4°C), and is thus suitable for use as a nanocarrier in the specific delivery of anticancer drugs to patients.

Keywords

anticancer therapy, doxorubicin-loaded apoferritin, encapsulation, long-term stability, protein nanocarriers

Authors

DOSTÁLOVÁ, S.; VAŠÍČKOVÁ, K.; HYNEK, D.; KŘÍŽKOVÁ, S.; RICHTERA, L.; VACULOVIČOVÁ, M.; ECKSCHLAGER, T.; STIBOROVÁ, M.; HEGER, Z.; ADAM, V.

Released

24. 3. 2017

Publisher

Dove Media Press

ISBN

1178-2013

Periodical

International Journal of Nanomedicine

Year of study

12

Number

1

State

New Zealand

Pages from

2265

Pages to

2278

Pages count

14

URL

https://www.dovepress.com/apoferritin-as-an-ubiquitous-nanocarrier-with-excellent-shelf-life-peer-reviewed-article-IJN

Full text in the Digital Library

http://hdl.handle.net/11012/69377

BibTex

@article{BUT134900,
  author="Simona {Dostálová} and Kateřina {Vašíčková} and David {Hynek} and Soňa {Křížková} and Lukáš {Richtera} and Markéta {Vaculovičová} and Tomáš {Eckschlager} and Marie {Stiborová} and Zbyněk {Heger} and Vojtěch {Adam}",
  title="Apoferritin as an ubiquitous nanocarrier with excellent shelf life",
  journal="International Journal of Nanomedicine",
  year="2017",
  volume="12",
  number="1",
  pages="2265--2278",
  doi="10.2147/IJN.S130267",
  issn="1178-2013",
  url="https://www.dovepress.com/apoferritin-as-an-ubiquitous-nanocarrier-with-excellent-shelf-life-peer-reviewed-article-IJN"
}