Přístupnostní navigace
E-application
Search Search Close
Publication detail
BAIAZITOVA, L. SKOPALÍK, J. SVOBODA, O. CHMELÍK, J. PROVAZNÍK, I.
Original Title
Fluorescence method for measuring cell proliferation by image analysis of cell confluency
Type
conference paper
Language
English
Original Abstract
This work is based on application image analysis for automatic measuring cell confluency of adherent cells labeled with fluorescent dyes. In this paper an example of segmentation of fibroblasts (3T3 Cells Line) with different levels of confluency is demonstrated. The proposed algorithm can be applied to modified culture chambers and different adherent cell lines. Precise cell enumeration and viability computing can help to optimize coating and culture protocol. These parameters are important for quality of migration or perfusion assay experiments in vitro.
Keywords
Confluency, segmentation, adherent cells, 3T3 Cells Line
Authors
BAIAZITOVA, L.; SKOPALÍK, J.; SVOBODA, O.; CHMELÍK, J.; PROVAZNÍK, I.
Released
30. 5. 2017
Publisher
Masarykova Univerzita - MUNI PRESS
Location
Brno, Czech Republic
ISBN
978-80-210-8580-0
Book
XVII. Workshop of Physical Chemists and Electrochemists
Edition
1
Edition number
Pages from
47
Pages to
48
Pages count
2
URL
http://www.sci.muni.cz/~labifel/files/soubory/sborn%C3%ADk_2017.pdf
BibTex
@inproceedings{BUT137050, author="Larisa {Chmelíková} and Josef {Skopalík} and Ondřej {Svoboda} and Jiří {Chmelík} and Valentine {Provazník}", title="Fluorescence method for measuring cell proliferation by image analysis of cell confluency", booktitle="XVII. Workshop of Physical Chemists and Electrochemists", year="2017", series="1", number="1", pages="47--48", publisher="Masarykova Univerzita - MUNI PRESS", address="Brno, Czech Republic", isbn="978-80-210-8580-0", url="http://www.sci.muni.cz/~labifel/files/soubory/sborn%C3%ADk_2017.pdf" }