Publication detail

Current trends in electrochemical sensing and biosensing of DNA methylation

KREJČOVÁ, L. RICHTERA, L. HYNEK, D. ADAM, V. LABUDA, J.

Original Title

Current trends in electrochemical sensing and biosensing of DNA methylation

Type

journal article in Web of Science

Language

English

Original Abstract

DNA methylation plays an important role in physiological and pathological processes. Several genetic diseases and most malignancies tend to be associated with aberrant DNA methylation. Among other analytical methods, electrochemical approaches have been successfully employed for characterisation of DNA methylation patterns that are essential for the diagnosis and treatment of particular diseases. This article discusses current trends in the electrochemical sensing and biosensing of DNA methylation. Particularly, it provides an overview of applied electrode materials, electrode modifications and biorecognition elements applications with an emphasis on strategies that form the core DNA methylation detection approaches. The three main strategies as (i) bisulfite treatment, (ii) cleavage by restriction endonucleases, and (iii) immuno/affinity reaction were described in greater detail. Additionally, the availability of the reviewed platforms for early cancer diagnosis and the approval of methylation inhibitors for anticancer therapy were discussed.

Keywords

Nucleic acids; DNA methylation; Sensor and biosensor; Electrochemistry; Cancer diagnosis

Authors

KREJČOVÁ, L.; RICHTERA, L.; HYNEK, D.; ADAM, V.; LABUDA, J.

Released

15. 11. 2017

ISBN

0956-5663

Periodical

BIOSENSORS & BIOELECTRONICS

Year of study

97

Number

1

State

Kingdom of the Netherlands

Pages from

384

Pages to

399

Pages count

16

URL

http://www.sciencedirect.com/science/article/pii/S0956566317300659

BibTex

@article{BUT137481,
  author="Ludmila {Krejčová} and Lukáš {Richtera} and David {Hynek} and Vojtěch {Adam} and Jan {Labuda}",
  title="Current trends in electrochemical sensing and biosensing of DNA methylation",
  journal="BIOSENSORS & BIOELECTRONICS",
  year="2017",
  volume="97",
  number="1",
  pages="384--399",
  doi="10.1016/j.bios.2017.06.004",
  issn="0956-5663",
  url="http://www.sciencedirect.com/science/article/pii/S0956566317300659"
}