Publication detail

Distinctive behaviour of live biopsy-derived carcinoma cells unveiled using coherence-controlled holographic microscopy

GÁL, B. VESELÝ, M. ČOLLÁKOVÁ, J. NEKULOVÁ, M. JŮZOVÁ, V. CHMELÍK, R. VESELÝ, P.

Original Title

Distinctive behaviour of live biopsy-derived carcinoma cells unveiled using coherence-controlled holographic microscopy

Type

journal article in Web of Science

Language

English

Original Abstract

Head and neck squamous cell carcinoma is one of the most aggressive tumours and is typically diagnosed too late. Late diagnosis requires an urgent decision on an effective therapy. An individualized test of chemosensitivity should quickly indicate the suitability of chemotherapy and radiotherapy. No ex vivo chemosensitivity assessment developed thus far has become a part of general clinical practice. Therefore, we attempted to explore the new technique of coherence-controlled holographic microscopy to investigate the motility and growth of live cells from a head and neck squamous cell carcinoma biopsy. We expected to reveal behavioural patterns characteristic for malignant cells that can be used to imrove future predictive evaluation of chemotherapy. We managed to cultivate primary SACR2 carcinoma cells from head and neck squamous cell carcinoma biopsy verified through histopathology. The cells grew as a cohesive sheet of suspected carcinoma origin, and western blots showed positivity for the tumour marker p63 confirming cancerous origin. Unlike the roundish colonies of the established FaDu carcinoma cell line, the SACR2 cells formed irregularly shaped colonies, eliciting the impression of the collective invasion of carcinoma cells. Time-lapse recordings of the cohesive sheet activity revealed the rapid migration and high plasticity of these epithelial-like cells. Individual cells frequently abandoned the swiftly migrating crowd by moving aside and crawling faster. The increasing mass of fast migrating epithelial-like cells before and after mitosis confirmed the continuation of the cell cycle. In immunofluorescence, analogously shaped cells expressed the p63 tumour marker, considered proof of their origin from a carcinoma. These behavioural traits indicate the feasible identification of carcinoma cells in culture according to the proposed concept of the carcinoma cell dynamic phenotype. If further developed, this approach could later serve in a new functional online analysis of reactions of carcinoma cells to therapy. Such efforts conform to current trends in precision medicine.

Keywords

carcinoma cells, coherence-controlled holographic microscopy

Authors

GÁL, B.; VESELÝ, M.; ČOLLÁKOVÁ, J.; NEKULOVÁ, M.; JŮZOVÁ, V.; CHMELÍK, R.; VESELÝ, P.

Released

28. 8. 2017

Publisher

PLOS

ISBN

1932-6203

Periodical

PLOS ONE

Year of study

12

Number

8

State

United States of America

Pages from

1

Pages to

14

Pages count

14

URL

http://europepmc.org/articles/PMC5573213?pdf=render

Full text in the Digital Library

http://hdl.handle.net/11012/84151

BibTex

@article{BUT138860,
  author="Břetislav {Gál} and Miroslav {Veselý} and Jana {Čolláková} and Marta {Nekulová} and Veronika {Jůzová} and Radim {Chmelík} and Pavel {Veselý}",
  title="Distinctive behaviour of live biopsy-derived carcinoma cells unveiled using coherence-controlled holographic microscopy",
  journal="PLOS ONE",
  year="2017",
  volume="12",
  number="8",
  pages="1--14",
  doi="10.1371/journal.pone.0183399",
  issn="1932-6203",
  url="http://europepmc.org/articles/PMC5573213?pdf=render"
}