Publication detail

Studying of quantum dot luminescence quenching effect caused by covalent conjugation with protein

PEJOVIČ SIMEUNOVIČ, J. PEKÁRKOVÁ, J. ŽÁK, J. CHAMRADOVÁ, I. HUBÁLEK, J.

Original Title

Studying of quantum dot luminescence quenching effect caused by covalent conjugation with protein

Type

journal article in Web of Science

Language

English

Original Abstract

The characterization of quantum dots (QDs) and their conjugates with bovine serum albumin was done using on-a-chip electrophoresis. Under optimal conditions, bioconjugated QDs were successfully separated from free QDs within approximate to10 min. Influence of coupling agent on a quenching of QD fluorescence intensity caused by conjugation to a protein was studied. When using two-step procedure combining 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide with N-hydroxysulfosuccinimide, the highest quenching effect was notable. We showed that QDs and capillary electrophoresis on a chip can be applied as a sensitive technique for the detection of biological molecules. The characteristics of this method are simplicity, small sample and reagent requirements, and high efficiency of separation.

Keywords

Bovine serum albumin Bioconjugation Electrophoresis Quantum dots Quenching effect

Authors

PEJOVIČ SIMEUNOVIČ, J.; PEKÁRKOVÁ, J.; ŽÁK, J.; CHAMRADOVÁ, I.; HUBÁLEK, J.

Released

14. 7. 2017

Publisher

Springer

Location

Vienna

ISBN

1434-4475

Periodical

Monatshefte für Chemie

Year of study

148

Number

11

State

Republic of Austria

Pages from

1901

Pages to

1909

Pages count

9

URL

https://link.springer.com/content/pdf/10.1007%2Fs00706-017-2038-1.pdf

BibTex

@article{BUT140291,
  author="Jelena {Pejović Simeunović} and Jana {Pekárková} and Jaromír {Žák} and Ivana {Chamradová} and Jaromír {Hubálek}",
  title="Studying of quantum dot luminescence quenching effect caused by covalent conjugation with protein

",
  journal="Monatshefte für Chemie",
  year="2017",
  volume="148",
  number="11",
  pages="1901--1909",
  doi="10.1007/s00706-017-2038-1",
  issn="1434-4475",
  url="https://link.springer.com/content/pdf/10.1007%2Fs00706-017-2038-1.pdf"
}