USE OF THE SILVER PHOSPHATE NANOPARTICLES (SPNPs) FOR THEIR ANTIMICROBIAL EFFECT ON BACTERIAL STRAINS

conference paper

English

Bacterial infections caused by Gram- or Gram(+) bacteria can be a serious complication in the treatment of many diseases. These strains are a common producer of nosocomial infections, which can be acquired in hospitals. The aim of many studies is to prevent these infections using various technical and technological procedures. For comparison of the antimicrobial effect of silver phosphate nanoparticles (SPNPs) in complexes with hyaluronic acid or chitosan on S. aureus and E. coli (8.0 * 10(7) CFU/1 ml), minimal inhibition concentration (MIC) was determined. SPNPs with typical spherical structure (200-300 nm) and compact structure were prepared. MIC of S. aureus with complex of hyaluronic acid with SPNPs resulted from the concentration of 25 mu M SPNPs and the total inhibition concentration (TIC) was 225 mu M SPNPs. MIC of S. aureus with complex of chitosan with SPNPs was 10 mu M SPNPs, and TIC was 25 mu M SPNPs. In the case of E. coli bacterial culture with complex of hyaluronic acid with SPNPs was MIC determined at 10 mu M SPNPs, and TIC was 150 mu M SPNPs. Applied complex of chitosan with SPNPs has MIC 10 mu M, which was the same as TIC. The best result was achieved at E. coli bacterial strain after the application of the complex of chitosan with SPNPs. Due to the increasing severity of the problem of resistance to commonly used antibiotic drugs, the study of the substances with antimicrobial effects, especially on the basis nanotechnologies, should be a priority in the future.

Silver phosphate nanoparticles; Hyaluronic acid; Chitosan; Antimicrobial effect

CHUDOBOVA, D.; ČÍHALOVÁ, K.; RUTTKAY-NEDECKÝ, B.; KOPEL, P.; ŽŮREK, M.; BASTL, K.; ADAM, V.; KIZEK, R.

1. 12. 2014

978-80-87294-47-5

NANOCON 2013

514

520

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