Přístupnostní navigace
E-application
Search Search Close
Publication detail
LOŠŤÁK, M. CHMELÍK, R.
Original Title
Coherence-controlled holographic microscopy principle embodiment into Q-PHASE microscope: story of a successful technology transfer
Type
conference paper
Language
English
Original Abstract
Curiously, the coherence-controlled holographic microscopy (CCHM) was brought into the world owing to the endeavor of Chmelik's team at Brno University of Technology (BUT) to avoid scanning in confocal microscopy. As coherence gating seemed to be the way, the Leith & Upatnieks proposal of incoherent holography had been considered attractive. Their method made interference system free from strict dependence on both spatial and temporal coherence. Off axis holographic system proposed on such basis has been proved capable of coherence based depth discrimination in single wide-field shot in reflected-light arrangement. Consequently, extremely low-coherence holographic imaging had been found highly contributive also to the image quality depriving it from coherence artefacts and improving its transversal resolution. This is why CCHM promised high precision of quantitative phase imaging (QPI) in transmitted light set up that was realized for cell biology. However the cost of necessarily complicated optical design and need of very precise mechanics forced the team of prof Chmelik at BUT to search for a company capable of mastering the instrument.
Keywords
holographic microscopy; quantitative phase imaging; coherence gate effect; live cells; turbid media; low coherence
Authors
LOŠŤÁK, M.; CHMELÍK, R.
Released
9. 3. 2016
ISBN
978-1-62841-952-8
Book
QUANTITATIVE PHASE IMAGING II
0277-786X
Periodical
Proceedings of SPIE
Year of study
9718
State
United States of America
Pages from
971816-1
Pages to
971816-6
Pages count
6
URL
https://www.spiedigitallibrary.org/conference-proceedings-of-spie/9718/1/Coherence-controlled-holographic-microscopy-principle-embodiment-into-Q-PHASE-microscope/10.1117/12.2225253.pdf?SSO=1