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HARTINGER, J. VESELÝ, P. NETÍKOVÁ, I. MATOUŠKOVÁ, E. PETRUŽELKA, L.
Original Title
The Protective Effect of Pyrimidine Nucleosides on Human HaCaT Keratinocytes Treated with 5-FU
Type
journal article in Web of Science
Language
English
Original Abstract
Background: Therapy with 5-fluorouracil (5-FU) and capecitabine is often complicated by skin toxicity (hand-foot syndrome, HFS). Topical application of uridine ointment is beneficial for alleviating HFS and other pyrimidine nucleosides have been described as 5-FU toxicity modulators. We tested pyrimidine nucleosides and their combinations to find the best combination for topical therapy of HFS. Materials and Methods: Cellular viability was measured by the real-time cell analyser and methyl thiazol tetrazolium (MTT) assay in order to evaluate the effect of pyrimidine nucleosides on HaCaT keratinocytes treated with 5-FU. The results were confirmed by evaluation of the cellular colonization by microphotography. Results: Cytidine and uridine protected keratinocytes to the same extent. Thymidine enhanced the protective effect when added to cytidine or uridine. Deoxycytidine did not have any protective effect. Conclusion: Our findings support the rationale for using uridine or cytidine in combination with thymidine in ointment for HFS treatment.
Keywords
Hand-foot syndrome; cytidine; uridine; thymidine; deoxycytidine; 5-FU; keratinocytes
Authors
HARTINGER, J.; VESELÝ, P.; NETÍKOVÁ, I.; MATOUŠKOVÁ, E.; PETRUŽELKA, L.
Released
1. 5. 2015
ISBN
0250-7005
Periodical
ANTICANCER RESEARCH
Year of study
35
Number
3
State
Hellenic Republic
Pages from
1303
Pages to
1310
Pages count
8
BibTex
@article{BUT142519, author="Jan {Hartinger} and Pavel {Veselý} and Irena {Štenglová Netíková} and Eva {Matoušková} and Luboš {Petruželka}", title="The Protective Effect of Pyrimidine Nucleosides on Human HaCaT Keratinocytes Treated with 5-FU", journal="ANTICANCER RESEARCH", year="2015", volume="35", number="3", pages="1303--1310", issn="0250-7005" }