Publication detail

Utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study

MÁROVÁ, I. SZOTKOWSKI, M. VANĚK, M. RAPTA, M. BYRTUSOVÁ, D. MIKHEICHYK, N. HÁRONIKOVÁ, A.

Original Title

Utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study

Type

journal article in Web of Science

Language

English

Original Abstract

Red yeast strains are ubiquitous microorganisms which accumulate substantial amounts of lipids and lipid-soluble metabolites. Red yeasts utilize many waste substrates of different origin. In this work red yeasts strains ( Rhodotorula, Sporobolomyces, Cystofilobasidium) were used for screening of growth and metabolic activity. As a carbon source waste animal fat and its hydrolysis products were used. Hydrolysis of animal fat was tested in alkaline as well as acidic conditions. As the substrate glucose (control), glycerol, crude animal fat, acid fat hydrolyzate and hydrolysate: glucose 1:2 were used. Screening of growth and metabolic activity of red yeasts was performed by flow cytometry. Extracellular lipase production was monitored as adaptation mechanism. Carotenoids, ergosterol and ubiquinone were quantified by HPLC/PDA/MS/ESI and the biomass was evaluated gravimetricaly.

Keywords

carotenogenic yeasts, lipids, animal waste

Authors

MÁROVÁ, I.; SZOTKOWSKI, M.; VANĚK, M.; RAPTA, M.; BYRTUSOVÁ, D.; MIKHEICHYK, N.; HÁRONIKOVÁ, A.

Released

17. 10. 2017

Publisher

European Biotechnology Thematic Network Association

Location

Editorial office, Piazza Università 1, Perugia, Italy

ISBN

2564-615X

Periodical

The EuroBiotech Journal

Year of study

1

Number

4

State

Republic of Italy

Pages from

310

Pages to

318

Pages count

9

URL

http://eurobiotechjournal.org/sayilar/93/buyuk/08_Res_Art_Marova.pdf

BibTex

@article{BUT143410,
  author="Ivana {Márová} and Martin {Szotkowski} and Martin {Vaněk} and Marek {Rapta} and Dana {Byrtusová} and Nadzeya {Mikheichyk} and Andrea {Němcová}",
  title="Utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study",
  journal="The EuroBiotech Journal",
  year="2017",
  volume="1",
  number="4",
  pages="310--318",
  doi="10.24190/ISSN2564-615X/2017/04.08",
  issn="2564-615X",
  url="http://eurobiotechjournal.org/sayilar/93/buyuk/08_Res_Art_Marova.pdf"
}