Publication detail

pH-responsive hybrid organic–inorganic ruthenium nanoparticles for controlled release of doxorubicin

BUCHTELOVÁ, H. STRMISKA, V. DOSTÁLOVÁ, S. MICHÁLEK, P. KŘÍŽKOVÁ, S. KOPEL, P. HYNEK, D. RICHTERA, L. ADAM, V. HEGER, Z.

Original Title

pH-responsive hybrid organic–inorganic ruthenium nanoparticles for controlled release of doxorubicin

Type

journal article in Web of Science

Language

English

Original Abstract

The current study aims at preparing biocompatible hybrid organic-inorganic ruthenium core-shell nanostructures (RuNPs) coated with polyvinylpyrrolidone (PVP) and polyoxyethylene stearate (POES). Thereafter, the core/shell RuNPs are loaded with doxorubicin (to form RuPDox) with a loading efficiency > 60%. RuPDox possesses exceptional stability and pH-responsive release kinetics with approx. 50% release of doxorubicin at up to 1 h exposure to an acidic endosomal environment. The cytotoxic effects of RuPDox are tested in vitro against breast cancer (MDA-MB-231), ovarian cancer (A2780), and neuroblastoma (UKF-NB-4) cells. Notably, although RuNPs have slight cytotoxicity only, RuPDox causes a synergistic enhancement of cytotoxicity when compared to free doxorubicin. Significant increase in free radicals formation, enhanced activity of executioner caspases 3/7, and higher expression of p53 and metallothionein is further identified due to the RuPDox treatment. Single-cell gel electrophoresis reveals no additional contribution of RuNPs to genotoxicity of doxorubicin. Moreover, RuPDox promotes significantly increased stability of doxorubicin in human plasma and pronounced hemocompatibility assayed on human red blood cells. The results imply a high potential of biocompatible hybrid RuNPs with PVP-POES shell as versatile nanoplatforms to enhance the efficiency of cancer treatment.

Keywords

biocompatibility; drug delivery; nanomedicine; polyoxyethylene stearate; polyvinylpyrrolidone

Authors

BUCHTELOVÁ, H.; STRMISKA, V.; DOSTÁLOVÁ, S.; MICHÁLEK, P.; KŘÍŽKOVÁ, S.; KOPEL, P.; HYNEK, D.; RICHTERA, L.; ADAM, V.; HEGER, Z.

Released

1. 11. 2017

ISBN

0934-0866

Periodical

PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION

Year of study

34

Number

11

State

Federal Republic of Germany

Pages from

1

Pages to

9

Pages count

9

BibTex

@article{BUT143490,
  author="Hana {Michálková} and Vladislav {Strmiska} and Simona {Dostálová} and Petr {Michálek} and Soňa {Křížková} and Pavel {Kopel} and David {Hynek} and Lukáš {Richtera} and Vojtěch {Adam} and Zbyněk {Heger}",
  title="pH-responsive hybrid organic–inorganic ruthenium nanoparticles for controlled release of doxorubicin",
  journal="PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION",
  year="2017",
  volume="34",
  number="11",
  pages="1--9",
  doi="10.1002/ppsc.201700289",
  issn="0934-0866"
}