ELECTROCHEMICAL DETECTION OF THE INTERACTION QUANTUM DOTS WITH METALLOTHIONEIN

conference paper

English

In the last period the interest of scientists is focused on nanoparticles (quantum dots, nanotubes, nanowires). Quantum dots (QDs) are widely studied. They can emit light radiation and from this reason they could be used like a fluorescence label for in vivo imaging. QDs can also bind proteins by unspecific binding. One from the most important protein in human body is metallothionein (MT), small cysteine-rich protein, which is responsible for binding of heavy metals, for accumulation of Zn, protection of cells to oxidative stress and it participates in the regulation of expression a number of major genes and enters to oxidative-reductive balance in a cell. From these reasons the interaction of MT with QD could play the important role at using QD in living organism. The study of this interaction is possible due to the electroactivity of both integrated components by electrochemical methods. The aim of this experiment was the study complexes MT-QDs created during the interaction of metallothionein with CuS QDs. Complexes determined by peaks Cat1, Cat2, RS2Co, Y and X were investigated in Brdicka´s solution by the differential pulse voltammetry on mercury electrode. The used interaction time was: 0 and 480 s; 30, 60, 90 min, and 2, 3, 4, 5 and 6 hrs. Brdicka´s solution was used as an electrolyte.

electrochemical detection; DPV, Brdicka´s reaction, metallothionein, quantum dot, MT-QD interaction

TMEJOVÁ, K.; KREMPLOVÁ, M.; JAROŠOVÁ, M.; HYNEK, D.; NEJDL, L.; KOPEL, P.; ADAM, V.; KIZEK, R.

21. 11. 2013

978-80-7375-908-7

MendelNet 2013: Proceedings of International PhD Students Conference

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