Publication detail

REAL-TIME SENSING OF DOXORUBICIN IN AN ISOLATED RAT HEART

BLAŽKOVÁ, I. VACULOVIČOVÁ, M. NOVÁKOVÁ, M. ADAM, V. KIZEK, R.

Original Title

REAL-TIME SENSING OF DOXORUBICIN IN AN ISOLATED RAT HEART

Type

conference paper

Language

English

Original Abstract

Doxorubixin is a highly effective and widely used antracycline antibiotic cytostatic drug used to treat numerous types of tumour diseases, but the cardiotoxic effect significantly limits its application. Doxorubicin has hot great fluorescence properties what can be used to its detection. The detection of the fluorescence of the therapeutics in organisms is limited by the thickness of the tissue the light need to penetrate. An alternative way for increasing the sensitivity of this type of imaging is the elimination of surrounding tissue, leading to ex vivo analysis under simulated conditions (i.e. perfusion system for isolated heart). The aim of this study was the combination of perfusion system with the fluorescence in vivo imaging system to observe the fluorescence compounds in beating heart. The study proposes the application of in vivo imaging system for fluorescence ex vivo analysis of rat heart from the doxorubicin administered rat. The miniaturized Langendorff perfusion system was used. The isolated heart was supply by oxygenated Tyrode solution (37 °C) to ensure the heart beating and nutrition. This arrangement enabled the detection of doxorubicin in the ex vivo heart. The detection concentration was 1 μg of doxorubicin in the heart after the intraperitoneal application of 4 mg doxorubicin.

Keywords

doxorubicin; fluorescence imaging; langendorff-perfused heart; cancer; magnetic particles

Authors

BLAŽKOVÁ, I.; VACULOVIČOVÁ, M.; NOVÁKOVÁ, M.; ADAM, V.; KIZEK, R.

Released

21. 11. 2013

ISBN

978-80-7375-908-7

Book

MENDELNET 2013

Pages from

691

Pages to

695

Pages count

5

BibTex

@inproceedings{BUT143941,
  author="Iva {Blažková} and Markéta {Vaculovičová} and Marie {Nováková} and Vojtěch {Adam} and René {Kizek}",
  title="REAL-TIME SENSING OF DOXORUBICIN IN AN ISOLATED RAT HEART",
  booktitle="MENDELNET 2013",
  year="2013",
  pages="691--695",
  isbn="978-80-7375-908-7"
}