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PUTEROVÁ, J. RAZUMOVA, O. MARTÍNEK, T. ALEXANDROV, O. DIVASHUK, M. KUBÁT, Z. HOBZA, R. KARLOV, G. KEJNOVSKÝ, E.
Original Title
Satellite DNA and Transposable Elements in Seabuckthorn ( Hippophae rhamnoides ), a Dioecious Plant with Small Y and Large X Chromosomes
Type
journal article in Web of Science
Language
English
Original Abstract
Seabuckthorn (Hippophae rhamnoides) is a dioecious shrub commonly used in the pharmaceutical, cosmetic, and environmental industry as a source of oil, minerals and vitamins. In this study, we analyzed the transposable elements and satellites in its genome.We carried out Illumina DNA sequencing and reconstructed the main repetitive DNA sequences. For data analysis, we developed a new bioinformatics approach for advanced satellite DNA analysis and showed that about 25% of the genome consists of satellite DNA and about 24% is formed of transposable elements, dominated by Ty3/Gypsy and Ty1/Copia LTR retrotransposons. FISH mapping revealed X chromosome-accumulated, Y chromosome-specific or both sex chromosomes-accumulated satellites but most satellites were found on autosomes. Transposable elements were located mostly in the subtelomeres of all chromosomes. The 5S rDNA and 45S rDNA were localized on one autosomal locus each. Although we demonstrated the small size of the Y chromosome of the seabuckthorn and accumulated satellite DNA there, we were unable to estimate the age and extent of the Y chromosome degeneration. Analysis of dioecious relatives such as Shepherdia would shed more light on the evolution of these sex chromosomes.
Keywords
sex chromosomes, genome composition, chromosomal localization, repetitive DNA
Authors
PUTEROVÁ, J.; RAZUMOVA, O.; MARTÍNEK, T.; ALEXANDROV, O.; DIVASHUK, M.; KUBÁT, Z.; HOBZA, R.; KARLOV, G.; KEJNOVSKÝ, E.
Released
2. 2. 2017
ISBN
1759-6653
Periodical
GENOME BIOL EVOL
Year of study
9
Number
1
State
United Kingdom of Great Britain and Northern Ireland
Pages from
197
Pages to
212
Pages count
16
URL
https://academic.oup.com/gbe/article/9/1/197/2830930
Full text in the Digital Library
http://hdl.handle.net/11012/137198
BibTex
@article{BUT144426, author="Janka {Puterová} and Olga {Razumova} and Tomáš {Martínek} and Oleg {Alexandrov} and Mikhail {Divashuk} and Zdeněk {Kubát} and Roman {Hobza} and Gennady {Karlov} and Eduard {Kejnovský}", title="Satellite DNA and Transposable Elements in Seabuckthorn ( Hippophae rhamnoides ), a Dioecious Plant with Small Y and Large X Chromosomes", journal="GENOME BIOL EVOL", year="2017", volume="9", number="1", pages="197--212", doi="10.1093/gbe/evw303", issn="1759-6653", url="https://academic.oup.com/gbe/article/9/1/197/2830930" }