Publication detail

Modulation of Induced Cytotoxicity of Doxorubicin by Using Apoferritin and Liposomal Cages

GUMULEC, J. FOJTŮ, M. RAUDENSKÁ, M. SZTALMACHOVÁ, M. SKOTÁKOVÁ, A. VLACHOVÁ, J. SKALIČKOVÁ, S. NEJDL, L. KOPEL, P. KNOPFOVÁ, L. ADAM, V. KIZEK, R. STIBOROVÁ, M. BABULA, P. MASAŘÍK, M.

Original Title

Modulation of Induced Cytotoxicity of Doxorubicin by Using Apoferritin and Liposomal Cages

Type

journal article in Web of Science

Language

English

Original Abstract

Doxorubicin is an effective chemotherapeutic drug, however, its toxicity is a significant limitation in therapy. Encapsulation of doxorubicin inside liposomes or ferritin cages decreases cardiotoxicity while maintaining anticancer potency. We synthesized novel apoferritin-and liposome-encapsulated forms of doxorubicin ("Apodox" and "lip-8-dox") and compared its toxicity with doxorubicin and Myocet on prostate cell lines. Three different prostatic cell lines PNT1A, 22Rv1, and LNCaP were chosen. The toxicity of the modified doxorubicin forms was compared to conventional doxorubicin using the MTT assay, real-time cell impedance-based cell growth method (RTCA), and flow cytometry. The efficiency of doxorubicin entrapment was 56% in apoferritin cages and 42% in the liposome carrier. The accuracy of the RTCA system was verified by flow-cytometric analysis of cell viability. The doxorubicin half maximal inhibition concentrations (IC50) were determined as 170.5, 234.0, and 169.0 nM for PNT1A, 22Rv1, and LNCaP, respectively by RTCA. Lip8-dox is less toxic on the non-tumor cell line PNT1A compared to doxorubicin, while still maintaining the toxicity to tumorous cell lines similar to doxorubicin or epirubicin (IC50 = 2076.7 nM for PNT1A vs. 935.3 and 729.0 nM for 22Rv1 and LNCaP). Apodox IC50 was determined as follows: 603.1, 1344.2, and 931.2 nM for PNT1A, 22Rv1, and LNCaP.

Keywords

doxorubicin; liposome; apoferritin; cancer; cardiotoxicity; modification; encapsulation

Authors

GUMULEC, J.; FOJTŮ, M.; RAUDENSKÁ, M.; SZTALMACHOVÁ, M.; SKOTÁKOVÁ, A.; VLACHOVÁ, J.; SKALIČKOVÁ, S.; NEJDL, L.; KOPEL, P.; KNOPFOVÁ, L.; ADAM, V.; KIZEK, R.; STIBOROVÁ, M.; BABULA, P.; MASAŘÍK, M.

Released

1. 12. 2014

Publisher

MDPI

ISBN

1422-0067

Periodical

International Journal of Molecular Sciences

Year of study

15

Number

12

State

Swiss Confederation

Pages from

22960

Pages to

22977

Pages count

18

URL

https://www.mdpi.com/1422-0067/15/12/22960

Full text in the Digital Library

http://hdl.handle.net/11012/189391

BibTex

@article{BUT145129,
  author="Jaromír {Gumulec} and Michaela {Fojtů} and Martina {Raudenská} and Markéta {Sztalmachová} and Anna {Skotáková} and Jana {Vlachová} and Sylvie {Skaličková} and Lukáš {Nejdl} and Pavel {Kopel} and Lucia {Knopfová} and Vojtěch {Adam} and René {Kizek} and Marie {Stiborová} and Petr {Babula} and Michal {Masařík}",
  title="Modulation of Induced Cytotoxicity of Doxorubicin by Using Apoferritin and Liposomal Cages",
  journal="International Journal of Molecular Sciences",
  year="2014",
  volume="15",
  number="12",
  pages="22960--22977",
  doi="10.3390/ijms151222960",
  issn="1422-0067",
  url="https://www.mdpi.com/1422-0067/15/12/22960"
}