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HEGER, Z. CERNEI, N. KUDR, J. GUMULEC, J. BLAŽKOVÁ, I. ZÍTKA, O. ECKSCHLAGER, T. STIBOROVÁ, M. ADAM, V. KIZEK, R.
Original Title
A Novel Insight into the Cardiotoxicity of Antineoplastic Drug Doxorubicin
Type
journal article in Web of Science
Language
English
Original Abstract
Doxorubicin is a commonly used antineoplastic agent in the treatment of many types of cancer. Little is known about the interactions of doxorubicin with cardiac biomolecules. Serious cardiotoxicity including dilated cardiomyopathy often resulting in a fatal congestive heart failure may occur as a consequence of chemotherapy with doxorubicin. The purpose of this study was to determine the effect of exposure to doxorubicin on the changes in major amino acids in tissue of cardiac muscle (proline, taurine, glutamic acid, arginine, aspartic acid, leucine, glycine, valine, alanine, isoleucine, threonine, lysine and serine). An in vitro interaction study was performed as a comparison of amino acid profiles in heart tissue before and after application of doxorubicin. We found that doxorubicin directly influences myocardial amino acid representation even at low concentrations. In addition, we performed an interaction study that resulted in the determination of breaking points for each of analyzed amino acids. Lysine, arginine, -alanine, valine and serine were determined as the most sensitive amino acids. Additionally we compared amino acid profiles of myocardium before and after exposure to doxorubicin. The amount of amino acids after interaction with doxorubicin was significantly reduced (p = 0.05). This fact points at an ability of doxorubicin to induce changes in quantitative composition of amino acids in myocardium. Moreover, this confirms that the interactions between doxorubicin and amino acids may act as another factor most likely responsible for adverse effects of doxorubicin on myocardium.
Keywords
myocardium; cardiomyopathy; interaction; amide bond; spectrophotometry; ion-exchange liquid chromatography
Authors
HEGER, Z.; CERNEI, N.; KUDR, J.; GUMULEC, J.; BLAŽKOVÁ, I.; ZÍTKA, O.; ECKSCHLAGER, T.; STIBOROVÁ, M.; ADAM, V.; KIZEK, R.
Released
1. 11. 2013
Publisher
MDPI
ISBN
1422-0067
Periodical
International Journal of Molecular Sciences
Year of study
14
Number
11
State
Swiss Confederation
Pages from
21629
Pages to
21646
Pages count
18
URL
https://www.mdpi.com/1422-0067/14/11/21629
Full text in the Digital Library
http://hdl.handle.net/11012/137222
BibTex
@article{BUT145130, author="Zbyněk {Heger} and Natalia Vladimirovna {Cernei} and Jiří {Kudr} and Jaromír {Gumulec} and Iva {Blažková} and Ondřej {Zítka} and Tomáš {Eckschlager} and Marie {Stiborová} and Vojtěch {Adam} and René {Kizek}", title="A Novel Insight into the Cardiotoxicity of Antineoplastic Drug Doxorubicin", journal="International Journal of Molecular Sciences", year="2013", volume="14", number="11", pages="21629--21646", doi="10.3390/ijms141121629", issn="1422-0067", url="https://www.mdpi.com/1422-0067/14/11/21629" }