Publication detail

Lead Ions Encapsulated in Liposomes and Their Effect on Staphylococcus aureus

KENŠOVÁ, R. BLAŽKOVÁ, I. KONEČNÁ, M. KOPEL, P. CHUDOBOVA, D. ZÍTKA, O. VACULOVIČOVÁ, M. HYNEK, D. ADAM, V. BEKLOVÁ, M. KIZEK, R.

Original Title

Lead Ions Encapsulated in Liposomes and Their Effect on Staphylococcus aureus

Type

journal article in Web of Science

Language

English

Original Abstract

The aim of the study was the preparation of a liposome complex with encapsulated lead ions, which were electrochemically detected. In particular, experiments were focused on the potential of using an electrochemical method for the determination of free and liposome-encapsulated lead and determination of the encapsulation efficiency preventing the lead toxicity. Primarily, encapsulation of lead ions in liposomes and confirmation of successful encapsulation by electrochemical methods was done. Further, the reduction effect of the liposome matrix on the detected electrochemical signal was monitored. Besides encapsulation itself, comparison of toxicity of free lead ions and lead ions encapsulated in liposome was tested. The calculated IC50 values for evaluating the lead cytotoxicity showed significant differences between the lead enclosed in liposomes (28 mu M) and free lead ions (237 mu M). From the cytotoxicity studies on the bacterial strain of S. aureus it was observed that the free lead ions are less toxic in comparison with lead encapsulated in liposomes. Liposomes appear to be a suitable carrier of various substances through the inner cavity. Due to the liposome structure the lead enclosed in the liposome is more easily accepted into the cell structure and the toxicity of the enclosed lead is higher in comparison to free lead ions.

Keywords

lead; liposome; toxicity; differential pulse voltammetry; cyclic voltammetry; atomic absorption spectrometry; IC50; Staphylococcus aureus

Authors

KENŠOVÁ, R.; BLAŽKOVÁ, I.; KONEČNÁ, M.; KOPEL, P.; CHUDOBOVA, D.; ZÍTKA, O.; VACULOVIČOVÁ, M.; HYNEK, D.; ADAM, V.; BEKLOVÁ, M.; KIZEK, R.

Released

1. 12. 2013

Publisher

MDPI

ISBN

1660-4601

Periodical

International Journal of Environmental Research and Public Health

Year of study

10

Number

12

State

Swiss Confederation

Pages from

6687

Pages to

6700

Pages count

14

URL

https://www.mdpi.com/1660-4601/10/12/6687

Full text in the Digital Library

http://hdl.handle.net/11012/181134

BibTex

@article{BUT145217,
  author="Renáta {Kenšová} and Iva {Blažková} and Marie {Konečná} and Pavel {Kopel} and Dagmar {chudobova} and Ondřej {Zítka} and Markéta {Vaculovičová} and David {Hynek} and Vojtěch {Adam} and Miroslava {Beklová} and René {Kizek}",
  title="Lead Ions Encapsulated in Liposomes and Their Effect on Staphylococcus aureus",
  journal="International Journal of Environmental Research and Public Health",
  year="2013",
  volume="10",
  number="12",
  pages="6687--6700",
  doi="10.3390/ijerph10126687",
  issn="1660-4601",
  url="https://www.mdpi.com/1660-4601/10/12/6687"
}