Publication detail

AUTOMATED ZINC PROTEINS EXTRACTION FROM PROSTATIC CANCER CELLS USING MAGNETIC PARTICLES

DOSTÁLOVÁ, S. JÍLKOVÁ, E. KŘÍŽKOVÁ, S. MASAŘÍK, M. ŠMERKOVÁ, K. HYNEK, D. RUTTKAY-NEDECKÝ, B. KREJČOVÁ, L. ADAM, V. KIZEK, R.

Original Title

AUTOMATED ZINC PROTEINS EXTRACTION FROM PROSTATIC CANCER CELLS USING MAGNETIC PARTICLES

Type

conference paper

Language

English

Original Abstract

Prostate cancer is a huge problem in modern world, mainly in the developed countries. It is important to diagnose this disease as soon as possible due to its high mortality. Prostate tissue has a unique ability to accumulate zinc ions. However cancer prostate tissue loses this ability. It is therefore possible to help the diagnosis of the prostate cancer via the isolation of zinc proteins contained in prostatic tissue. This isolation can be carried out using magnetic particles modified with anti-Zn-II-antibodies. Our objective was to create a procedure for isolation of zinc proteins from prostatic cells using magnetic particles and automated pipetting station. Cell lines PNT1A and LNCaP were used for experiments. Cell line PNT1A represents healthy prostate tissue, line LNCaP represent cancer tissue. There was decreasing level (10%) of Zn-II ions observed in the cancer prostatic cell lines for the 25 and 50 mu M concentrations of zinc.

Keywords

electrochemistry; magnetic particles; prostate cancer; Zn-II

Authors

DOSTÁLOVÁ, S.; JÍLKOVÁ, E.; KŘÍŽKOVÁ, S.; MASAŘÍK, M.; ŠMERKOVÁ, K.; HYNEK, D.; RUTTKAY-NEDECKÝ, B.; KREJČOVÁ, L.; ADAM, V.; KIZEK, R.

Released

1. 1. 2012

ISBN

978-80-7375-836-3

Book

MENDELNET 2012

Pages from

1174

Pages to

1182

Pages count

9

BibTex

@inproceedings{BUT145371,
  author="Simona {Dostálová} and Eva {Jílková} and Soňa {Křížková} and Michal {Masařík} and Kristýna {Šmerková} and David {Hynek} and Branislav {Ruttkay-Nedecký} and Ludmila {Krejčová} and Vojtěch {Adam} and René {Kizek}",
  title="AUTOMATED ZINC PROTEINS EXTRACTION FROM PROSTATIC CANCER CELLS USING MAGNETIC PARTICLES",
  booktitle="MENDELNET 2012",
  year="2012",
  pages="1174--1182",
  isbn="978-80-7375-836-3"
}