Publication detail

ELECTROCHEMICAL DETECTION OF QUANTUM DOTS USED AS A TOOL FOR LABELLING INFLUENZA DERIVED OLIGONUCLEOTIDE

KREJČOVÁ, L. HYNEK, D. KOPEL, P. ADAM, V. HUBÁLEK, J. KIZEK, R.

Original Title

ELECTROCHEMICAL DETECTION OF QUANTUM DOTS USED AS A TOOL FOR LABELLING INFLUENZA DERIVED OLIGONUCLEOTIDE

Type

conference paper

Language

English

Original Abstract

The aim of this study was designed hybridization assay consisting of probe modified magnetic particles (MPs) and specific oligonucleotide labeled by quantum dots (QDs), and their application in the detection of avian influenza virus (H5N1) respectively influenza derived oligonucleotide (ODN). There were two basic steps used in the assay: (i) isolation of specific oligonucleotide (target) by anti-sense bounded MPs and (ii) target detection by QDs marker. It was optimized the efficiency of hybridization temperatures between anti senses and target influenza oligonucleotide. QDs labeled target was detected by electrochemical analysis. Our results show that MPs based automatic isolation together with electrochemical detection is available tool for influenza detection.

Keywords

voltammetry; quantum dots; influenza; oligonucleotide; nanoparticles; automatic isolation hybridization assay; electrochemistry

Authors

KREJČOVÁ, L.; HYNEK, D.; KOPEL, P.; ADAM, V.; HUBÁLEK, J.; KIZEK, R.

Released

22. 11. 2012

ISBN

978-80-7375-836-3

Book

MENDELNET 2012

Pages from

1233

Pages to

1243

Pages count

11

BibTex

@inproceedings{BUT145625,
  author="Ludmila {Krejčová} and David {Hynek} and Pavel {Kopel} and Vojtěch {Adam} and Jaromír {Hubálek} and René {Kizek}",
  title="ELECTROCHEMICAL DETECTION OF QUANTUM DOTS USED AS A TOOL FOR LABELLING INFLUENZA DERIVED OLIGONUCLEOTIDE",
  booktitle="MENDELNET 2012",
  year="2012",
  pages="1233--1243",
  isbn="978-80-7375-836-3"
}