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NEJDL, L. ROZEKOVÁ, P. VACULOVIČOVÁ, M. KUDR, J. RUTTKAY-NEDECKÝ, B. KOPEL, P. STIBOROVÁ, M. ECKSCHLAGER, T. ADAM, V. KIZEK, R.
Original Title
CADMIUM TELLURIDE QUANTUM DOTS AS FLUORESCENT PROBE FOR DETERMINATION OF VALPROIC ACID
Type
conference paper
Language
English
Original Abstract
Valproate (VPA, valproic acid, C8H16O2) belongs to the pharmacological class of antiepileptics of the 2nd generation [1]. VPA is a neuropsychiatric drug with a very broad clinical use. Broad therapeutic activity of VPA is mainly due to the action of neurotransmitters, transcription factors and gene expression [2]. Recently, it has become evident that VPA is also associated with anti-cancer activity [3]. The VPA and some VPA-analogues inhibit histonedeacetylases class I and IIa and modulate the biology of diverse tumour cell entities by inducing differentiation, inhibiting proliferation, increasing apoptosis by decreasing metastatic and angiogenetic potential [4]. The anti-tumour characteristics of VPA have been demonstrated [3]. Therefore, it is necessary to determinate the VPA in biological samples. There is a variety of techniques for the VPA detection, e.g.: ultra-performance liquid chromatographic system coupled with tandem mass spectrometry [5], high-performance liquid chromatography with electrochemical, fluorescence, diode array and/or tandem mass spectrometric detection [6-9], gas chromatography with flame ionization or electron-capture detection [10, 11], capillary electrophoresis with contactless conductivity or indirect UV detection [12, 13]. These techniques are precise but demanding sophisticated instrumentation and operator training. Therefore, we study a fast and simple method for detection of VPA based on changes in fluorescence intensity of CdTe quantum dots (QDs). It was demonstrated that VPA prevents quenching of CdTe QDs after addition of KCl. This finding can be used for sensitive and selective analysis of VPA.
Keywords
CdTe quantum dots; fluorescence; Valproate (VPA, valproic acid)
Authors
NEJDL, L.; ROZEKOVÁ, P.; VACULOVIČOVÁ, M.; KUDR, J.; RUTTKAY-NEDECKÝ, B.; KOPEL, P.; STIBOROVÁ, M.; ECKSCHLAGER, T.; ADAM, V.; KIZEK, R.
Released
1. 1. 2015
ISBN
978-80-904959-3-7
Book
CECE 2015
Pages from
153
Pages to
156
Pages count
4
BibTex
@inproceedings{BUT145681, author="NEJDL, L. and ROZEKOVÁ, P. and VACULOVIČOVÁ, M. and KUDR, J. and RUTTKAY-NEDECKÝ, B. and KOPEL, P. and STIBOROVÁ, M. and ECKSCHLAGER, T. and ADAM, V. and KIZEK, R.", title="CADMIUM TELLURIDE QUANTUM DOTS AS FLUORESCENT PROBE FOR DETERMINATION OF VALPROIC ACID", booktitle="CECE 2015", year="2015", pages="153--156", isbn="978-80-904959-3-7" }