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ZALEWSKA, M. KŘÍŽKOVÁ, S. SIERADZKA, S. TMEJOVÁ, K. MERLOS RODRIGO, M. HYNEK, D. ADAM, V. KIZEK, R. MILNEROWICZ, H.
Original Title
Analysis of human and rabbit metallothioneins by Brdicka reaction and mass spectrometry
Type
abstract
Language
English
Original Abstract
Metallothioneins (MTs) form a large family of evolutionarily conserved low-molecular-weight proteins (~6 kDa), found in practically all life forms, in vertebrates, invertebrates, fungi and even in plants. MT plays a special role in maintaining the homeostasis of metals essential for the proper functioning of the human body, including Zn, Cu. MT is also responsible for detoxification of heavy metals such as Cd and Hg and removal of free radicals. Mammalian MTs were separated into two charge-separable isoforms, designated as MT fractions 1 and 2. The six amino acids of rabbit MT sequence show marked differences compared with the sequences of other mammalian MTs. The biological significance for this difference remains unclear. The present study demonstrates an analytical approach of employing two detection techniques: Brdicka reaction and matrix-assisted laser desorption/ionization - mass spectrometry analysis (MALDI-MS) to characterize MTs from human and rabbit liver.
Keywords
Brdicka reaction; mass spectrometry; metallothionein
Authors
ZALEWSKA, M.; KŘÍŽKOVÁ, S.; SIERADZKA, S.; TMEJOVÁ, K.; MERLOS RODRIGO, M.; HYNEK, D.; ADAM, V.; KIZEK, R.; MILNEROWICZ, H.
Released
1. 9. 2014
ISBN
1742-464X
Periodical
FEBS Journal
Year of study
281
Number
1
State
United Kingdom of Great Britain and Northern Ireland
Pages from
318
Pages to
Pages count
BibTex
@misc{BUT145895, author="ZALEWSKA, M. and KŘÍŽKOVÁ, S. and SIERADZKA, S. and TMEJOVÁ, K. and MERLOS RODRIGO, M. and HYNEK, D. and ADAM, V. and KIZEK, R. and MILNEROWICZ, H.", title="Analysis of human and rabbit metallothioneins by Brdicka reaction and mass spectrometry", year="2014", journal="FEBS Journal", volume="281", number="1", pages="318--318", issn="1742-464X", note="abstract" }