Publication detail

Analysis of human and rabbit metallothioneins by Brdicka reaction and mass spectrometry

ZALEWSKA, M. KŘÍŽKOVÁ, S. SIERADZKA, S. TMEJOVÁ, K. MERLOS RODRIGO, M. HYNEK, D. ADAM, V. KIZEK, R. MILNEROWICZ, H.

Original Title

Analysis of human and rabbit metallothioneins by Brdicka reaction and mass spectrometry

Type

abstract

Language

English

Original Abstract

Metallothioneins (MTs) form a large family of evolutionarily conserved low-molecular-weight proteins (~6 kDa), found in practically all life forms, in vertebrates, invertebrates, fungi and even in plants. MT plays a special role in maintaining the homeostasis of metals essential for the proper functioning of the human body, including Zn, Cu. MT is also responsible for detoxification of heavy metals such as Cd and Hg and removal of free radicals. Mammalian MTs were separated into two charge-separable isoforms, designated as MT fractions 1 and 2. The six amino acids of rabbit MT sequence show marked differences compared with the sequences of other mammalian MTs. The biological significance for this difference remains unclear. The present study demonstrates an analytical approach of employing two detection techniques: Brdicka reaction and matrix-assisted laser desorption/ionization - mass spectrometry analysis (MALDI-MS) to characterize MTs from human and rabbit liver.

Keywords

Brdicka reaction; mass spectrometry; metallothionein

Authors

ZALEWSKA, M.; KŘÍŽKOVÁ, S.; SIERADZKA, S.; TMEJOVÁ, K.; MERLOS RODRIGO, M.; HYNEK, D.; ADAM, V.; KIZEK, R.; MILNEROWICZ, H.

Released

1. 9. 2014

ISBN

1742-464X

Periodical

FEBS Journal

Year of study

281

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

318

Pages to

318

Pages count

1

BibTex

@misc{BUT145895,
  author="ZALEWSKA, M. and KŘÍŽKOVÁ, S. and SIERADZKA, S. and TMEJOVÁ, K. and MERLOS RODRIGO, M. and HYNEK, D. and ADAM, V. and KIZEK, R. and MILNEROWICZ, H.",
  title="Analysis of human and rabbit metallothioneins by Brdicka reaction and mass spectrometry",
  year="2014",
  journal="FEBS Journal",
  volume="281",
  number="1",
  pages="318--318",
  issn="1742-464X",
  note="abstract"
}