Publication result detail

Spectrophotometric Assay of Aflatoxin B1 Using Acetylcholinesterase Immobilized on Standard Microplates

POHANKA, M.

Original Title

Spectrophotometric Assay of Aflatoxin B1 Using Acetylcholinesterase Immobilized on Standard Microplates

English Title

Spectrophotometric Assay of Aflatoxin B1 Using Acetylcholinesterase Immobilized on Standard Microplates

Type

WoS Article

Original Abstract

Aflatoxins are a group of hepatotoxic secondary metabolites produced by molds Aspergillus. During inappropriate storage or processing of food and beverages, aflatoxins can be distributed and become a serious health problem. Disparate analytical techniques were prepared for the assay in the past. Here, a method based on inhibition of enzyme acetylcholinesterase (AChE) is performed allowing fast and simple prove of aflatoxins presence. For the assay purposes, AChE was immobilized on standard polystyrene microplates using gelatin as a stabilizing substance. Both free and immobilized AChEs were used and compared one to each other. Immobilization protocol was optimized and interference of organic solvents such as methanol was tested. For the immobilized AChE, limit of detection 2.75 ppb for aflatoxin B1 was received. The immobilized AChE kept full activity at least one month. Suitability of the assay for a practical performance is considered.

English abstract

Aflatoxins are a group of hepatotoxic secondary metabolites produced by molds Aspergillus. During inappropriate storage or processing of food and beverages, aflatoxins can be distributed and become a serious health problem. Disparate analytical techniques were prepared for the assay in the past. Here, a method based on inhibition of enzyme acetylcholinesterase (AChE) is performed allowing fast and simple prove of aflatoxins presence. For the assay purposes, AChE was immobilized on standard polystyrene microplates using gelatin as a stabilizing substance. Both free and immobilized AChEs were used and compared one to each other. Immobilization protocol was optimized and interference of organic solvents such as methanol was tested. For the immobilized AChE, limit of detection 2.75 ppb for aflatoxin B1 was received. The immobilized AChE kept full activity at least one month. Suitability of the assay for a practical performance is considered.

Keywords

Acetylcholinesterase; Aflatoxin; Aspergillus; Biosensor; Butyrylcholinesterase; Ellman method; Mycotoxin

Key words in English

Acetylcholinesterase; Aflatoxin; Aspergillus; Biosensor; Butyrylcholinesterase; Ellman method; Mycotoxin

Authors

POHANKA, M.

RIV year

2018

Released

24.05.2013

ISBN

0003-2719

Periodical

ANALYTICAL LETTERS

Volume

46

Number

8

State

United States of America

Pages from

1306

Pages to

1315

Pages count

10

BibTex

@article{BUT145991,
  author="Miroslav {Pohanka}",
  title="Spectrophotometric Assay of Aflatoxin B1 Using Acetylcholinesterase Immobilized on Standard Microplates",
  journal="ANALYTICAL LETTERS",
  year="2013",
  volume="46",
  number="8",
  pages="1306--1315",
  doi="10.1080/00032719.2012.757703",
  issn="0003-2719"
}