Publication detail

Immobilization of ligninolytic enzymes from white-rot fungi in cross-linked aggregates

VOBĚRKOVÁ, S. SOLČÁNY, V. VRŠANSKÁ, M. ADAM, V.

Original Title

Immobilization of ligninolytic enzymes from white-rot fungi in cross-linked aggregates

Type

journal article in Web of Science

Language

English

Original Abstract

Ligninolytic enzymes from white-rot fungi are widely used in biotechnological processes. However, the application of these enzymes as free enzymes is limited due to their instability and lack of reusability. Enzyme stabilization is therefore a major challenge in biocatalytic process research, and immobilization methods are desirable. Using cross-linked enzyme aggregates (CLEAs) such as magnetic CLEAs, porous-CLEAs and combi-CLEAs is a promising technique for overcoming these issues. Cross-linking methods can stabilize and immobilize enzymes by interconnecting enzyme molecules via multiple bonds using cross linking agents such as glutaraldehyde. The high catalyst density and microporous assembly of CLEAs guarantee high catalyst activity, which, together with their long shelf life, operational stability, and reusability, provide a cost-efficient alternative to matrix-assisted immobilization approaches. Here, we review current progress in ligninolytic enzyme immobilization and provide a comprehensive review of CLEAs. Moreover, we summarize the use of these CLEAs for biocatalysis processes, bioremediation such as dye decolourization, wastewater treatment or pharmaceutically active compound elimination.

Keywords

White-rot fungi; Ligninolytic enzymes; Cross-linked enzyme aggregates; Immobilization

Authors

VOBĚRKOVÁ, S.; SOLČÁNY, V.; VRŠANSKÁ, M.; ADAM, V.

Released

1. 7. 2018

ISBN

0045-6535

Periodical

CHEMOSPHERE

Year of study

202

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

694

Pages to

707

Pages count

14

BibTex

@article{BUT147398,
  author="Stanislava {Voběrková} and Veronika {Solčány} and Martina {Vršanská} and Vojtěch {Adam}",
  title="Immobilization of ligninolytic enzymes from white-rot fungi in cross-linked aggregates",
  journal="CHEMOSPHERE",
  year="2018",
  volume="202",
  number="1",
  pages="694--707",
  doi="10.1016/j.chemosphere.2018.03.088",
  issn="0045-6535"
}