NEW FLUORESCENCE LABELING STRATEGY BY STIMULI RESPONSIVE QUANTUM DOTS

abstract

English

Substances migrating over the capillary in capillary electrophoresis (CE) may be affected by various physical stimuli without the need for large adjustments of the device. In particular, it is a whole range of lasers (laser diodes or classical LEDs) of different wavelengths and intensity. In addition, the capillary can be tempered usually in the range of 20-60 °C or influenced by the magnetic field by magnet. As a result, the capillary can be used as a nonreactor for preparations (synthesis) different substances that can be induced with irradiation, temperature, microwaves, magnetic and/or electric fields. Then the resulting product may be conjugated with biomolecules/ organics dye or others stimuli responsive materials and separated by electroosmotic flow. The novelty of this work is based on activatable or stimuli-responsive design of the CdTe quantum dots for fluorescence libeling of biomolecules driven by CE. Proposed quantum dots can be activated/synthesized by temperatures in the range of 25-90 °C or UV-irradiation. Even though the toxic materials (cadmium and potassium borohydride) were used for synthesis, the proposed method can be labelled as “environmentally friendly” because the process (synthesis of QDs and protein conjugation) was carried out under mild conditions: ultra-low volume (nanoliter scale), relatively low temperature (50 °C), low pressure (atmospheric pressure), and in short time (under 90 seconds). This study opens up new possibilities for utilization of classical CE in synthesis of nanoparticles and on-line labeling biomolecules in nanoliter scale and in short time.

Capillary electrophoresis; stimuli responsive quantum dots

NEJDL, L.; ADAM, V.; VACULOVIČOVÁ, M.

18. 5. 2018

978-88-941816-1-6

42nd International Symposium on Capillary Chromatography and 15th GC×GC Symposium ABSTRACT BOOK

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