Publication detail

Influence of liposomal platinum cytostatics on cancer cells by voltammetric methods

SEDLÁČKOVÁ, E. LANÍKOVÁ, P. HYNEK, D. KREJČOVÁ, L. BUCHTELOVÁ, H. RICHTERA, L. ADAM, V.

Original Title

Influence of liposomal platinum cytostatics on cancer cells by voltammetric methods

Type

conference paper

Language

English

Original Abstract

Aim of this work deals with closing of cisplatin into liposomes, the study of their stability and interaction with cancer cell lines. Commercial cisplatin cytostatic was encapsulated into three types of liposomes (L8, L10 and L15) of a different chemical composition. Each of liposomes had different ability to close cytostatic. L8 differs from L10 by containing cholesterol. In despite of L10 and L8, L15 liposome contains a synthetic 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (C41H78NO8P) – DOPE, which is generally found in cell membranes. The size of the liposomes was characterized by Dynamic Light Scattering (DLS). The amount of Pt-drug in liposomes was verified by electrochemical determination. Their stability was observed by differential pulse voltammetry (DPV). These studies also demonstrated, that the cisplatin is stable in physiological solution for at least 24 hours, which making it a suitable medium storage for this drug. Finally, the encapsulated cisplatin was applied to cancer cell lines and their viability was observed by MTT assay.

Keywords

Cisplatin; liposomes; electrochemistry; differential pulse voltammetry; cancer cell lines

Authors

SEDLÁČKOVÁ, E.; LANÍKOVÁ, P.; HYNEK, D.; KREJČOVÁ, L.; BUCHTELOVÁ, H.; RICHTERA, L.; ADAM, V.

Released

31. 3. 2018

Publisher

TANGER

ISBN

978-80-87294-81-9

Book

NANOCON 2017

Edition

1st Edition, 2018

Pages from

548

Pages to

553

Pages count

6

URL

https://nanocon2017.tanger.cz/cz/

Full text in the Digital Library

http://hdl.handle.net/11012/178375

BibTex

@inproceedings{BUT148684,
  author="Eliška {Dostálová} and Petra {Laníková} and David {Hynek} and Ludmila {Krejčová} and Hana {Michálková} and Lukáš {Richtera} and Vojtěch {Adam}",
  title="Influence of liposomal platinum cytostatics on cancer cells by voltammetric methods",
  booktitle="NANOCON 2017",
  year="2018",
  series="1st Edition, 2018",
  pages="548--553",
  publisher="TANGER",
  isbn="978-80-87294-81-9",
  url="https://nanocon2017.tanger.cz/cz/"
}