Publication detail

Sarcosine degradation pathway is involved in the epigenetics of prostate cells

STRMISKA, V. MICHÁLEK, P. BUCHTELOVÁ, H. LACKOVÁ, Z. GURÁŇ, R. KŘÍŽKOVÁ, S. POMPEIANO VANÍČKOVÁ, L. ZÍTKA, O. STIBOROVÁ, M. ECKSCHLAGER, T. ADAM, V. HEGER, Z.

Original Title

Sarcosine degradation pathway is involved in the epigenetics of prostate cells

Type

conference paper

Language

English

Original Abstract

It has been shown that sarcosine suplementation stimulates the proliferation of prostate cells and also their invassiveness. In present study we show that enzymes conected with sarcosine conversion to glycine (sarcosine dehydrogenase, pipecolic acid oxidase) are stimulated due to sarcosine treatment. Further, sarcosine treatment increases S-adenosylmethioneine-to-S-adenosylhomocysteine ratio, which indicates a release and utilization of free methyl groups from sarcosine degradation pathway. We identified the highest induction of global methylation in non-malignant PNT1A cells, but global methylation profiles were altered also in malignant (22Rv1) and metastatic (LNCaP) cells. The influence on methylation changes was further verified using hypomethylating agent 5-azacytidine (5-aza). Co-treatment of prostate cells with 5-aza and sarcosine resulted in decrease in cells invassiveness when compared to treatment with sarcosine alone. This correlates with sarcosine-related hypermethylation of genes involved in cells growth and cell cycle.

Keywords

Sarcosine; methylation; prostate; prostate cancer; human cells

Authors

STRMISKA, V.; MICHÁLEK, P.; BUCHTELOVÁ, H.; LACKOVÁ, Z.; GURÁŇ, R.; KŘÍŽKOVÁ, S.; POMPEIANO VANÍČKOVÁ, L.; ZÍTKA, O.; STIBOROVÁ, M.; ECKSCHLAGER, T.; ADAM, V.; HEGER, Z.

Released

31. 12. 2017

Publisher

Mendel University in Brno

ISBN

978-80-7509-529-9

Book

MendelNet 2017

Pages from

927

Pages to

931

Pages count

5

URL

https://mendelnet.cz/artkey/mnt-201701-0108_Sarcosine-degradation-pathway-is-involved-in-the-epigenetics-of-prostate-cells.php?back=/magno/mnt/2017/mn1.php?secid=4

Full text in the Digital Library

http://hdl.handle.net/11012/84186

BibTex

@inproceedings{BUT148758,
  author="Vladislav {Strmiska} and Petr {Michálek} and Hana {Michálková} and Zuzana {Lacková} and Roman {Guráň} and Soňa {Křížková} and Lucie {Pompeiano Vaníčková} and Ondřej {Zítka} and Marie {Stiborová} and Tomáš {Eckschlager} and Vojtěch {Adam} and Zbyněk {Heger}",
  title="Sarcosine degradation pathway is involved in the epigenetics of prostate cells",
  booktitle="MendelNet 2017",
  year="2017",
  pages="927--931",
  publisher="Mendel University in Brno",
  isbn="978-80-7509-529-9",
  url="https://mendelnet.cz/artkey/mnt-201701-0108_Sarcosine-degradation-pathway-is-involved-in-the-epigenetics-of-prostate-cells.php?back=/magno/mnt/2017/mn1.php?secid=4"
}