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TOLDE, O. GANDALOVIČOVÁ, A. KŘÍŽOVÁ, A. VESELÝ, P. CHMELÍK, R. RÖSEL, D. BRÁBEK, J.
Original Title
Quantitative phase imaging unravels new insight into dynamics of mesenchymal and amoeboid cancer cell invasion
Type
journal article in Web of Science
Language
English
Original Abstract
Observation and analysis of cancer cell behaviour in 3D environment is essential for full understanding of the mechanisms of cancer cell invasion. However, label-free imaging of live cells in 3D conditions is optically more challenging than in 2D. Quantitative phase imaging provided by coherence controlled holographic microscopy produces images with enhanced information compared to ordinary light microscopy and, due to inherent coherence gate effect, enables observation of live cancer cells’ activity even in scattering milieu such as the 3D collagen matrix. Exploiting the dynamic phase differences method, we for the first time describe dynamics of differences in cell mass distribution in 3D migrating mesenchymal and amoeboid cancer cells, and also demonstrate that certain features are shared by both invasion modes. We found that amoeboid fibrosarcoma cells’ membrane blebbing is enhanced upon constriction and is also occasionally present in mesenchymally invading cells around constricted nuclei. Further, we demonstrate that both leading protrusions and leading pseudopods of invading fibrosarcoma cells are defined by higher cell mass density. In addition, we directly document bundling of collagen fibres by protrusions of mesenchymal fibrosarcoma cells. Thus, such a non-invasive microscopy offers a novel insight into cellular events during 3D invasion.
Keywords
Quantitative phase imaging , holographic microscopy, cancer cell invasion
Authors
TOLDE, O.; GANDALOVIČOVÁ, A.; KŘÍŽOVÁ, A.; VESELÝ, P.; CHMELÍK, R.; RÖSEL, D.; BRÁBEK, J.
Released
13. 8. 2018
Publisher
Springer Nature
ISBN
2045-2322
Periodical
Scientific Reports
Number
8
State
United Kingdom of Great Britain and Northern Ireland
Pages from
1
Pages to
13
Pages count
URL
http://link.springer.com/article/10.1038/s41598-018-30408-7
Full text in the Digital Library
http://hdl.handle.net/11012/84157
BibTex
@article{BUT149217, author="Ondřej {Tolde} and Aneta {Gandalovičová} and Aneta {Křížová} and Pavel {Veselý} and Radim {Chmelík} and Daniel {Rösel} and Jan {Brábek}", title="Quantitative phase imaging unravels new insight into dynamics of mesenchymal and amoeboid cancer cell invasion", journal="Scientific Reports", year="2018", number="8", pages="1--13", doi="10.1038/s41598-018-30408-7", issn="2045-2322", url="http://link.springer.com/article/10.1038/s41598-018-30408-7" }