Přístupnostní navigace
E-application
Search Search Close
Publication detail
PONOMAREV, V. SUKHORUKOVA, I. SHEVEYKO, A. PERMYAKOVA, E. MANAKHOV, A. IGNATOV, S. GLOUSHANKOVA, N. ZHITNYAK, I. LEBEDEV, O. POLČÁK, J. KOZMIN, A. SHTANSKY, D.
Original Title
Antibacterial Performance of TiCaPCON Films Incorporated with Ag, Pt, and Zn: Bactericidal Ions Versus Surface Microgalvanic Interactions
Type
journal article in Web of Science
Language
English
Original Abstract
is very important to prevent bacterial colonization at the early postoperative stages. There are four major strategies and their corresponding types of antibacterial surfaces specifically designed to fight infection: bactericide release, anti-adhesion, pHsensitive, and contact-killing. Herein, we aimed at determining the antibacterial efficiency of different types of bactericidal ions and revealing the possible contribution of surface microgalvanic effects arising from a potential difference on heterogeneous surfaces. We considered five types of TiCaPCON films, with Ag, Zn, Pt, Ag + Zn, and Pt + Zn nanoparticles (NPs) on their surface. The Ag-modified film demonstrated a pronounced antibacterial effect at a very low Ag ion concentration of 0.11 ppb in physiological solution that was achieved already after 3 h of immersion in Escherichia coli (E. coli) bacterial culture. The Zn-containing sample also showed a noticeable antibacterial effect against E. coli and Staphylococcus aureus (S. aureus) strains, wherein the concentration of Zn ions was 2 orders of magnitude higher (15 ppb) compared with the Ag ions. The presence of Ag NPs accelerated the leaching of Zn ion out of the TiCaPCON−Ag−Zn film, but no synergistic effect of the simultaneous presence of the two bactericidal components was observed. After the incubation of the samples with Ag, Zn, and Ag + Zn NPs in E. coli and S. aureus suspensions for 24 and 8 h, respectively, all bacterial cells were completely inactivated. The Pt-containing film showed a very low Pt ion release, and therefore the contribution of this type of ions to the total bactericidal effect could be neglected. The results of the electrochemical studies and Kelvin probe force microscopy indicated that microgalvanic couples were formed between the Pt NPs and the TiCaPCON film, but no noticeable antibacterial effect against either E. coli or S. aureus strains was observed. All ion-modified samples provided good osteoblastic cell attachment, spreading, and proliferation and therefore were concluded to be nontoxic for cells. In addition, the TiCaPCON films with Ag, Pt, and Zn NPs on their surface demonstrated good osteoconductive characteristics.
Keywords
antibacterial films, bactericide ion release, electrochemical behavior, Kelvin probe force microscopy, microgalvanic effect, cytocompatibility
Authors
PONOMAREV, V.; SUKHORUKOVA, I.; SHEVEYKO, A.; PERMYAKOVA, E.; MANAKHOV, A.; IGNATOV, S.; GLOUSHANKOVA, N.; ZHITNYAK, I.; LEBEDEV, O.; POLČÁK, J.; KOZMIN, A.; SHTANSKY, D.
Released
25. 7. 2018
ISBN
1944-8244
Periodical
ACS APPL MATER INTER
Year of study
10
Number
29
State
United States of America
Pages from
24406
Pages to
24420
Pages count
15
URL
https://pubs-acs-org.ezproxy.lib.vutbr.cz/doi/abs/10.1021/acsami.8b06671
BibTex
@article{BUT149892, author="Vladimir {Ponomarev} and I.V. {Sukhorukova} and A.N. {Sheveyko} and Elizaveta {Permyakova} and Anton {Manakhov} and Sergei G. {Ignatov} and Natalia A. {Gloushankova} and I.Y. {Zhitnyak} and Oleg {Lebedev} and Josef {Polčák} and A.M. {Kozmin} and Dmitry V. {Shtansky}", title="Antibacterial Performance of TiCaPCON Films Incorporated with Ag, Pt, and Zn: Bactericidal Ions Versus Surface Microgalvanic Interactions", journal="ACS APPL MATER INTER", year="2018", volume="10", number="29", pages="24406--24420", doi="10.1021/acsami.8b06671", issn="1944-8244", url="https://pubs-acs-org.ezproxy.lib.vutbr.cz/doi/abs/10.1021/acsami.8b06671" }