Publication detail

Upconversion nanoparticle bioconjugates characterized by capillary electrophoresis

VANĚČKOVÁ, T. ŠMERKOVÁ, K. ZÍTKA, J. HYNEK, D. ZÍTKA, O. HLAVÁČEK, A. FORET, F. ADAM, V. VACULOVIČOVÁ, M.

Original Title

Upconversion nanoparticle bioconjugates characterized by capillary electrophoresis

Type

journal article in Web of Science

Language

English

Original Abstract

Upconversion nanoparticles (UCNPs) are an emerging class of optical materials with high potential in bioimaging due to practically no background signal and high penetration depth. Their excellent optical properties and easy surface functionalization make them perfect for conjugation with targeting ligands. In this work, capillary electrophoretic (CE) method with laser-induced fluorescence detection was used to investigate the behavior of carboxyl-silica-coated UCNPs. Folic acid, targeting folate receptor overexpressed by wide variety of cancer cells, was used for illustrative purposes and assessed by CE under optimized conditions. Peptide-mediated bioconjugation of antibodies to UCNPs was also investigated. Despite the numerous advantages of CE, this is the first time that CE was employed for characterization of UCNPs and their bioconjugates. The separation conditions were optimized including the background electrolyte concentration and pH. The optimized electrolyte was 20 mM borate buffer with pH 8.

Keywords

Laser diode; Near-infrared excitation; Photon upconversion; Separation method

Authors

VANĚČKOVÁ, T.; ŠMERKOVÁ, K.; ZÍTKA, J.; HYNEK, D.; ZÍTKA, O.; HLAVÁČEK, A.; FORET, F.; ADAM, V.; VACULOVIČOVÁ, M.

Released

1. 9. 2018

ISBN

0173-0835

Periodical

Electrophoresis

Year of study

39

Number

17

State

Federal Republic of Germany

Pages from

2246

Pages to

2252

Pages count

7

BibTex

@article{BUT149924,
  author="Tereza {Vaněčková} and Kristýna {Šmerková} and Jan {Zítka} and David {Hynek} and Ondřej {Zítka} and Antonín {Hlaváček} and František {Foret} and Vojtěch {Adam} and Markéta {Vaculovičová}",
  title="Upconversion nanoparticle bioconjugates characterized by capillary electrophoresis",
  journal="Electrophoresis",
  year="2018",
  volume="39",
  number="17",
  pages="2246--2252",
  doi="10.1002/elps.201700483",
  issn="0173-0835"
}