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TESAŘOVÁ M., MANCINI L., SIMON A., ADAMEYKO I., KAUCKÁ M., ELEWA A., LANZAFAME G., ZHANG Y., KALASOVÁ D., SZAROWSKÁ B., ZIKMUND T., NOVOTNÁ M., KAISER J.
Original Title
A quantitative analysis of 3D-cell distribution in regenerating muscle-skeletal system with synchrotron X-ray computed microtomography
Type
journal article in Web of Science
Language
English
Original Abstract
One of the greatest enigmas of modern biology is how the geometry of muscular and skeletal structures are created and how their development is controlled during growth and regeneration. Scaling and shaping of vertebrate muscles and skeletal elements has always been enigmatic and required an advanced technical level in order to analyse the cell distribution in 3D. In this work, synchrotron X-ray computed microtomography (μCT) and chemical contrasting has been exploited for a quantitative analysis of the 3D-cell distribution in tissues of a developing salamander (Pleurodeles waltl) limb – a key model organism for vertebrate regeneration studies. We mapped the limb muscles, their size and shape as well as the number and density of cells within the extracellular matrix of the developing cartilage. By using tomographic approach, we explored the polarity of the cells in 3D, in relation to the structure of developing joints. We found that the polarity of chondrocytes correlates with the planes in joint surfaces and also changes along the length of the cartilaginous elements. Our approach generates data for the precise computer simulations of muscle-skeletal regeneration using cell dynamics models, which is necessary for the understanding how anisotropic growth results in the precise shapes of skeletal structures.
Keywords
CT, tomography, 3D, cell, salamander
Authors
Released
20. 9. 2018
Publisher
Springer Nature
ISBN
2045-2322
Periodical
Scientific Reports
Year of study
8
Number
1
State
United Kingdom of Great Britain and Northern Ireland
Pages from
Pages to
13
Pages count
12
URL
http://link.springer.com/article/10.1038/s41598-018-32459-2
Full text in the Digital Library
http://hdl.handle.net/11012/85329
BibTex
@article{BUT150099, author="Markéta {Kaiser} and Dominika {Kalasová} and Tomáš {Zikmund} and Marie {Novotná} and Jozef {Kaiser}", title="A quantitative analysis of 3D-cell distribution in regenerating muscle-skeletal system with synchrotron X-ray computed microtomography", journal="Scientific Reports", year="2018", volume="8", number="1", pages="1--13", doi="10.1038/s41598-018-32459-2", issn="2045-2322", url="http://link.springer.com/article/10.1038/s41598-018-32459-2" }