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GUSZPIT, E. KOPEL, P. KŘÍŽKOVÁ, S. MILNEROWICZ, H.
Original Title
Preliminary analysis of the interactions between CdTe quantum dots and human metallothionein
Type
journal article in Web of Science
Language
English
Original Abstract
Metallothionein (MT) plays the important role in the detoxification of heavy metals, protection against oxidative compounds and as a prognostic marker in the development of tumors. It is important to find selective, stable and sensitive tools and probes to evaluate the presence of MT in biological fluids or tissues. QDs linked with ligands such as peptides or small molecules are a promising tool for selective, fast, and sensitive tagging and imaging in medicine. In previous findings, the authors proved the possibility of interaction with QDs (particularly with CdTe) and analyzed the stability of the formed complexes between CdTe and MT during incubation over time. Following that, an initial analysis of the interactions between CdTe quantum dots (QDs) and human metallothionein (MT) was performed. Complexes of mercaptosuccinic acid-covered CdTe QDs + MT were investigated using fluorescence intensity changes along a timeline, quenching analysis, stability interpretation based on zeta potential, and quenching intensity. Based on the preliminary results, it appears as though the possible interactions depend on the size of the CdTe QDs. Additionally, the formation of complexes between CdTe and human MT likely depends mostly on structural changes and conformational reorganization rather than on electrostatic interactions. Both types of interactions are responsible for complex creation and stabilization.
Keywords
Quantum dots; Metallothionein; Bioconjugation; Interactions; Biomarkers
Authors
GUSZPIT, E.; KOPEL, P.; KŘÍŽKOVÁ, S.; MILNEROWICZ, H.
Released
1. 10. 2018
ISBN
0927-7765
Periodical
Colloids and Surfaces B: Biointerfaces
Year of study
170
Number
1
State
Kingdom of the Netherlands
Pages from
447
Pages to
453
Pages count
7
BibTex
@article{BUT150569, author="Ewelina {Guszpit} and Pavel {Kopel} and Soňa {Křížková} and Halina {Milnerowicz}", title="Preliminary analysis of the interactions between CdTe quantum dots and human metallothionein", journal="Colloids and Surfaces B: Biointerfaces", year="2018", volume="170", number="1", pages="447--453", doi="10.1016/j.colsurfb.2018.06.045", issn="0927-7765" }