Publication detail

Comparison of cation-exchange capillary columns used for ion chromatographic separation of biogenic amines

LI, Y. NESTERENKO, P. STANLEY, R. PAULL, B. MACKA, M.

Original Title

Comparison of cation-exchange capillary columns used for ion chromatographic separation of biogenic amines

Type

journal article in Web of Science

Language

English

Original Abstract

The selectivity for 15 biogenic amines and amino acids shown by three capillary cation-exchange columns, lonPac CS19, CS12A and CS17 (250 x 0.4 mm ID, all from Thermo Fisher Scientific), exhibiting medium, medium low and ultra-low hydrophobicity, and either carboxylic or mixed carboxyliciphosphonic acid functional groups, was investigated. A mixed mode retention mechanism was revealed with ion exchange, hydrophobic and hydrogen bonding interactions contributing to retention of polar organic molecules on these phases. The relative impact of these interactions was evaluated via the effect of concentration and pH of the eluent (methanesulfonic acid) on the retention of fifteen structurally similar biogenic amines and amino acids. Strong hydrogen bonding interactions were observed between the solute amino acid carboxylic groups and cation-exchange groups from the ion-exchangers. This is the first time retention data correlated with logP data has revealed clustering of the solutes in two groups, according to the presence or absence of a carboxylic acid functional group. In addition, stronger retention behaviour was found for the lonPac CS12A cation-exchanger, containing both carboxylic and phosphonic functional groups. Further assessment of the orthogonality plots of retention factors for the three stationary phases revealed that the columns exhibited different complimentary selectivity that can be utilised to achieve specific separations.

Keywords

Capillary liquid chromatography; Mixed-mode retention; Ethylvinylbenzene-divinylbenzene based; ion-exchange resin; Cation-exchange stationary phase; Retention mechanism; Hydrophobic and hydrogen bonding interactions

Authors

LI, Y.; NESTERENKO, P.; STANLEY, R.; PAULL, B.; MACKA, M.

Released

12. 10. 2018

ISBN

0021-9673

Periodical

Journal of Chromatography A

Year of study

1571

Number

1

State

Kingdom of the Netherlands

Pages from

193

Pages to

200

Pages count

8

BibTex

@article{BUT150585,
  author="Yan {Li} and Pavel {Nesterenko} and Roger {Stanley} and Brett {Paull} and Miroslav {Macka}",
  title="Comparison of cation-exchange capillary columns used for ion chromatographic separation of biogenic amines",
  journal="Journal of Chromatography A",
  year="2018",
  volume="1571",
  number="1",
  pages="193--200",
  doi="10.1016/j.chroma.2018.08.021",
  issn="0021-9673"
}