Publication detail

Adaptation of Cupriavidus necator H16 to levulinic acid for enhancement of 3-hydroxyvalerate content in copolymer P(3HB-co-3HV)

NOVÁČKOVÁ, I. KUČERA, D. POŘÍZKA, J. PERNICOVÁ, I. SEDLÁČEK, P. OBRUČA, S.

Original Title

Adaptation of Cupriavidus necator H16 to levulinic acid for enhancement of 3-hydroxyvalerate content in copolymer P(3HB-co-3HV)

Type

abstract

Language

English

Original Abstract

Within this work we used methods of evolutionary engineering to gain strains adapted to levulinic acid able to growth at the presence of levulinic acid and to convert it to 3HV and incorporate into copolymer P(3HB-co-3HV) more than wild-type strain. On the basis of specific enzyme activities of selected enzymes through metabolism of evolved strains we tried to suggest possible metabolic adaptation strategies and we also used methods of statistical analysis such as PCA and AHC for searching of common and different strategies between evolved strains.

Keywords

evolutionary engineering, levulinic acid, P(3HB-co-3HV)

Authors

NOVÁČKOVÁ, I.; KUČERA, D.; POŘÍZKA, J.; PERNICOVÁ, I.; SEDLÁČEK, P.; OBRUČA, S.

Released

13. 11. 2018

Location

Brno

ISBN

978-80-210-9069-9

Book

XIX. setkání biochemiků a molekulárních biologů Wimmerová, Michaela, Holková, Jitka, eds.

Pages from

76

Pages to

76

Pages count

1

BibTex

@misc{BUT151417,
  author="Ivana {Nováčková} and Dan {Kučera} and Jaromír {Pořízka} and Iva {Buchtíková} and Petr {Sedláček} and Stanislav {Obruča}",
  title="Adaptation of Cupriavidus necator H16 to levulinic acid for enhancement of 3-hydroxyvalerate content in copolymer P(3HB-co-3HV)",
  booktitle="XIX. setkání biochemiků a molekulárních biologů Wimmerová, Michaela, Holková, Jitka, eds.",
  year="2018",
  pages="76--76",
  address="Brno",
  isbn="978-80-210-9069-9",
  note="abstract"
}