Effects of hydration of sodium 3-hydroxybutyrate on phase transitions connected to cryoprotectivity of microorganisms

abstract

English

In this work, we are focused on cryoprotective mechanisms utilized by bacterial strain Cupriavidus necator H16 which produces and accumulates polyhydroxyalkanoates (PHA) in form of intracellular granules because of storage of carbon and energy and innate ability to protect itself against stress conditions. But intracellular granules do not obtain only PHA, due to metabolism of the PHA where PHA is simultaneously synthesized and degraded, the granules also contain its monomer 3-hydroxybutyrate (3HB).In our experimental part, we used differential scanning calorimetry (DSC) to observe the glass transitions and separated them into one independent and two dependent regions of phase diagram on water content. Also using the DSC data, we established the amount of nonfreezing water, which was calculated from linear regression (Figure 1) to be approx. 1,22g/g of monomer Na3HB. For determination of sorption isotherms of Na3HB with various content of water, we measured water activity by sorption calorimetry, dynamic vapor sorption and Novasina LabMaster which correspond to data obtained from DSC.

sodium 3-hydroxybutyrate, cryoprotectant, water activity, phase diagram, DSC

SLANINOVÁ, E.; SEDLÁČEK, P.; OBRUČA, S.; KOCHERBITOV, V.

12. 9. 2018

Vysoké učení technické v Brně, Fakulta chemická

Purkyňova 464/118, 612 00 Brno

978-80-214-5488-0

7th Meeting on Chemistry and Life 2018. Book of abstracts

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