Publication detail

Polyhadroxyalkanoates –microbial polyesters with a complex protective effect against diverse stress factors

SLANINOVÁ, E. SEDLÁČEK, P. OBRUČA, S.

Original Title

Polyhadroxyalkanoates –microbial polyesters with a complex protective effect against diverse stress factors

Type

abstract

Language

English

Original Abstract

Polyhydroxyalkanoates (PHAs) are hydrophobic polyesters produced and accumulated by numerous microorganisms in a form of intracellular granules filled with the polymer and covered by a layer of proteins. The polymer content of bacteria can reach up to 90 % of the weight of cellular dry matter. In our recent work, we have confirmed experimentally that PHAs granules do not serve only as a source of carbon or energy but also as a cell component with a complex protective performance. PHAs accumulating bacteria cells are synthesizing and degrading the polymers simultaneously. We have confirmed that the monomer of PHAs, which is found in the polymer-accumulating bacterial in a substantial amount, serves as a chemical chaperone and a cryo- protective solute. Furthermore, we have revealed that the protective function of the polymer against various stressors such as non-optimal pH, high and low temperature, osmotic up- and down-shock, UV exposure etc. is conditioned by the native amorphous state of PHAs. This liquid-like state of PHAs, found only in-vivo, is involved in the adaptation of cells supporting them to survive sudden changes of the environment.

Keywords

PHA, stress factors, protectants, intracellular granule

Authors

SLANINOVÁ, E.; SEDLÁČEK, P.; OBRUČA, S.

Released

13. 11. 2018

Publisher

Tiskárna Didot, spol. s. r. o.

Location

Trnkova 119, 628 00 Brno-Líšeň

ISBN

978-80-210-9069-9

Book

XIX. setkání biochemiků a molekulárních biologů

Pages from

44

Pages to

44

Pages count

103

BibTex

@misc{BUT151441,
  author="Eva {Slaninová} and Petr {Sedláček} and Stanislav {Obruča}",
  title="Polyhadroxyalkanoates –microbial polyesters with a complex protective effect against diverse stress factors",
  booktitle="XIX. setkání biochemiků a molekulárních biologů",
  year="2018",
  pages="44--44",
  publisher="Tiskárna Didot, spol. s. r. o.",
  address="Trnkova 119, 628 00 Brno-Líšeň",
  isbn="978-80-210-9069-9",
  note="abstract"
}