Publication detail

In vivo efficiency of antimicrobial inorganic bone grafts in osteomyelitis treatments

MESTRES, G. FERNANDEZ-YAGUE, M. A. PASTORINO, D. MONTUFAR JIMENEZ, E. CANAL, C. MANZANARES-CESPEDES, M. C. GINEBRA, M. P.

Original Title

In vivo efficiency of antimicrobial inorganic bone grafts in osteomyelitis treatments

Type

journal article in Web of Science

Language

English

Original Abstract

The purpose of the present work was to evaluate in vivo different antimicrobial therapies to eradicate osteomyelitis created in the femoral head of New Zealand rabbits. Five phosphate-based cements were evaluated: calcium phosphate cements (CPC) and calcium phosphate foams (CPF), both in their pristine form and loaded with doxycycline hyclate, and an intrinsic antimicrobial magnesium phosphate cement (MPC; not loaded with an antibiotic). The cements were implanted in a bone previously infected with Staphylococcus aureus to discern the effects of the type of antibiotic administration (systemic vs. local), porosity (microporosity, i.e. < 5 μm vs. macroporosity, i.e. > 5 μm) and type of antimicrobial mechanism (release of antibiotic vs. intrinsic antimicrobial activity) on the improvement of the health state of the infected animals. A new method was developed, with a more comprehensive composite score that integrates 5 parameters of bone infection, 4 parameters of bone structural integrity and 4 parameters of bone regeneration. This method was used to evaluate the health state of the infected animals, both before and after osteomyelitis treatment. The results showed that the composite score allows to discern statistically significant differences between treatments that individual evaluations were not able to identify. Despite none of the therapies completely eradicated the infection, it was observed that macroporous materials (CPF and CPFd, the latter loaded with doxycycline hyclate) and intrinsic antimicrobial MPC allowed a better containment of the osteomyelitis. This study provides novel insights to understand the effect of different antimicrobial therapies in vivo, and a promising comprehensive methodology to evaluate the health state of the animals was developed. We expect that the implementation of such methodology could improve the criteria to select a proper antimicrobial therapy.

Keywords

Osteomyelitis; Calcium phosphate cements; Calcium phosphate foams; Magnesium phosphate cements; Drug delivery; In vivo

Authors

MESTRES, G.; FERNANDEZ-YAGUE, M. A.; PASTORINO, D.; MONTUFAR JIMENEZ, E.; CANAL, C.; MANZANARES-CESPEDES, M. C. ; GINEBRA, M. P.

Released

1. 4. 2019

Publisher

Elsevier

Location

Nizozemsko

ISBN

0928-4931

Periodical

Materials Science and Engineering C-Materials for Biological Applications

Number

C97

State

Kingdom of the Netherlands

Pages from

84

Pages to

95

Pages count

12

URL

https://www-sciencedirect-com.ezproxy.lib.vutbr.cz/science/article/pii/S0928493118308646

BibTex

@article{BUT151790,
  author="MESTRES, G. and FERNANDEZ-YAGUE, M. A. and PASTORINO, D. and MONTUFAR JIMENEZ, E. and CANAL, C. and MANZANARES-CESPEDES, M. C. and GINEBRA, M. P.",
  title="In vivo efficiency of antimicrobial inorganic bone grafts in osteomyelitis treatments",
  journal="Materials Science and Engineering C-Materials for Biological Applications",
  year="2019",
  number="C97",
  pages="84--95",
  doi="10.1016/j.msec.2018.11.064",
  issn="0928-4931",
  url="https://www-sciencedirect-com.ezproxy.lib.vutbr.cz/science/article/pii/S0928493118308646"
}