Publication detail

Utilization of analytical instrumental methods in phytotoxicity testing of nanoparticles

MODLITBOVÁ, P. NOVOTNÝ, K. POŘÍZKA, P. FARKA, Z. ZLÁMALOVÁ GARGOŠOVÁ, H. ROMIH, T. KAISER, J.

Original Title

Utilization of analytical instrumental methods in phytotoxicity testing of nanoparticles

Type

conference proceedings

Language

English

Original Abstract

In this work, we focused on several analytical instrumental methods utilization and the benefits of their outcomes in phytotoxicity assessment. Two model plants - Allium cepa L. and Lemna minor L. were used to test five types of cadmium Quantum Dots (QDs). In the first study, L. minor was used to test cadmium telluride QDs capped with two coating ligands: glutathione (GSH) or 3-mercaptopropionic acid (MPA). Macroscopic toxicity endpoints number of fronds and fresh weight of L. minor were determined after 168-hour exposure and growth rate inhibition and final biomass inhibition were evaluated. The next objective was to determine total content of Cd in plants using Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES). Laser-Induced Breakdown Spectroscopy (LIBS) with 200 µm lateral resolution was employed to obtain two-dimensional maps of Cd spatial distribution in L. minor fronds. Finally, L. minor fronds were subjected to transmission electron microscopy (TEM) to examine QDs ability to penetrate cell walls. In the A. cepa phytotoxicity study, cadmium telluride QDs with or without shells were tested, i.e. 2-mercaptopropionic acid capped CdTe, CdTe/ZnS, and CdTe/CdS/ZnS QDs. CdCl2 served as positive control. After 24-hour and 72-hour exposure, the total length of the root system was measured. Then the total Cd content in different plant tissues (roots, bulbs, and shoots) was determined by ICP-OES. A. cepa roots were examined by fluorescence microscopy to determine adsorption of Cd-based QDs on the root surface and its relation to the QD type. Free Cd2+ content in exposure media before and after the test was measured by differential pulse voltametry (DPV) to assess differences in chemical stability among the QD types.

Keywords

cadmium; quantum dots; phytotoxicity; bioaccumulation

Authors

MODLITBOVÁ, P.; NOVOTNÝ, K.; POŘÍZKA, P.; FARKA, Z.; ZLÁMALOVÁ GARGOŠOVÁ, H.; ROMIH, T.; KAISER, J.

Released

19. 6. 2018

ISBN

9788409037995

Book

Book of abstracts of the ISEAC‐40 Internaonal Conference on Environmental & Food  Monitoring

Pages from

380

Pages to

380

Pages count

442

BibTex

@proceedings{BUT151872,
  editor="MODLITBOVÁ, P. and NOVOTNÝ, K. and POŘÍZKA, P. and FARKA, Z. and ZLÁMALOVÁ GARGOŠOVÁ, H. and ROMIH, T. and KAISER, J.",
  title="Utilization of analytical instrumental methods in phytotoxicity testing of nanoparticles",
  year="2018",
  pages="380--380",
  isbn="9788409037995"
}