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SVOBODOVÁ, Z. KUPČÍK, R. SMĚLÁ, D. ROUPEC, J. MACHÁČEK, O. NOVÁKOVÁ, L. BÍLKOVÁ, Z.
Original Title
ELECTROMAGNET FOR CAPTURE OF MAGNETIC BEADS FROM LARGE VOLUME INTO MICROFLUIDIC CHAMBRE CHIP
Type
conference paper
Language
English
Original Abstract
Immunomagnetic separation is method of choice besides filtration or centrifugation when a bioanalyte is to be specifically isolated form a larger volume. Here, home-made electromagnet with special, optimized construction, to fit to the microfluidic rhombic chip is presented. The experiments show how to capture 1 mg of superparamagnetic beads (SMB) from 20 mL syringe into 120 µL chamber in microfluidic chip. The captured beads can be easily transported to another microfluidic device for subsequent analysis. Various flow rates were tested and SMB were collected firstly from a phosphate buffer and subsequently from a cow's milk sample.
Keywords
electromagnet, magnetic beads, microfluidic, chip
Authors
SVOBODOVÁ, Z.; KUPČÍK, R.; SMĚLÁ, D.; ROUPEC, J.; MACHÁČEK, O.; NOVÁKOVÁ, L.; BÍLKOVÁ, Z.
Released
16. 10. 2018
Publisher
Institute of Analytical Chemistry of the CAS
Location
Brno
ISBN
978-80-904959-5-1
Book
CECE 2018
Edition
15th Internation Interdisciplinary Meeting on Bioanalysis
Pages from
286
Pages to
289
Pages count
4
BibTex
@inproceedings{BUT153354, author="SVOBODOVÁ, Z. and KUPČÍK, R. and SMĚLÁ, D. and ROUPEC, J. and MACHÁČEK, O. and NOVÁKOVÁ, L. and BÍLKOVÁ, Z.", title="ELECTROMAGNET FOR CAPTURE OF MAGNETIC BEADS FROM LARGE VOLUME INTO MICROFLUIDIC CHAMBRE CHIP", booktitle="CECE 2018", year="2018", series="15th Internation Interdisciplinary Meeting on Bioanalysis", pages="286--289", publisher="Institute of Analytical Chemistry of the CAS", address="Brno", isbn="978-80-904959-5-1" }