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Publication detail
Haoqing Zhang, Ying Xu, Zdenka Fohlerova, Honglong Chang, Ciprian Iliescu, Pavel Neuzil
Original Title
LAMP-on-a-Chip: Revising Microfluidic Platforms for Loop-Mediated DNA Amplification
Type
journal article in Web of Science
Language
English
Original Abstract
Nucleic acid amplification for the detection of infectious diseases, food pathogens, or assessment of genetic disorders require a laboratory setting with specialized equipment and technical expertise. Isothermal deoxyribonucleic acid amplification methods, such as loop-mediated isothermal amplification (LAMP), exhibit characteristics ideal for point-of-care (POC) applications, since their instrumentation is less demanding in comparison with the standard method of polymerase chain reaction. Other key advantages of LAMP are robustness and the production of pyrophosphate in the presence of the target gene, enabling to detect the reaction products using the naked eye. Polymerase inhibitors, presented in clinical samples, do not affect the amplification process, making LAMP suitable for a simple sample-to-answer diagnostic systems with simplified sample preparation. In this review, we discuss the trends in miniaturized LAMP techniques, such as microfluidic, paper-based, and digital with their advantages and disadvantages, especially for POC applications alongside our opinion of the future development of miniaturized LAMP.
Keywords
PCR; LAMP; chip; microfluidics
Authors
Released
31. 1. 2019
ISBN
0165-9936
Periodical
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
Number
5
State
United Kingdom of Great Britain and Northern Ireland
Pages from
44
Pages to
53
Pages count
10
BibTex
@article{BUT154078, author="Zdenka {Fohlerová} and Pavel {Neužil}", title="LAMP-on-a-Chip: Revising Microfluidic Platforms for Loop-Mediated DNA Amplification ", journal="TRAC-TRENDS IN ANALYTICAL CHEMISTRY", year="2019", number="5", pages="44--53", doi="10.1016/j.trac.2019.01.015", issn="0165-9936" }